1.Immobilized Anti-ROR1 (Zilovertamab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human ROR1 Protein (His Tag) (Cat#TMPY-04649), the EC50 is 3.0-9.0 ng/mL(QC tested).
2.Loaded Anti-ROR1 (Zilovertamab Biosimilar) can bind Recombinant Human ROR1 Protein, His Tag (Cat#TMPY-04649) with an affinity constant of 19.3 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
3.Immobilized Recombinant Human ROR1 Protein (His Tag) (Cat#TMPY-04649) at 1 μg/mL (100 μL/well) can bind Anti-ROR1 (Zilovertamab Biosimilar), the EC50 is 0.8 ng/mL (Routinely tested).
4.Anti-ROR1 (Zilovertamab Biosimilar) captured on Protein A chip, can bind Recombinant Human ROR1 Protein (His Tag) (Cat#TMPY-04649) with an affinity constant of 88.420 nM as determined in an SPR assay (Biacore T200) (Routinely tested).

ROR1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 43.4 kDa and the accession number is Q01973.

Human

HEK293 Cells

receptor tyrosine kinase-like orphan receptor 1,NTRKR1,dJ537F10.1

A DNA sequence encoding the human ROR1 (NP_005003.2) (Met1-Glu403) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 30

≥ 95% as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.