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5T4/TPBG Protein, Cynomolgus, Recombinant (His & Myc)

(Synonyms: Wnt-activated inhibitory factor 1 (WAIF1), Trophoblast glycoprotein, TPBG) Copy Product Info

Synonyms: Wnt-activated inhibitory factor 1 (WAIF1), Trophoblast glycoprotein, TPBG

Catalog No. TMPH-02437 Copy Product Info
May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. 5T4/TPBG Protein, Cynomolgus, Recombinant (His & Myc) is expressed in HEK293 mammalian cells with N-10xHis and C-Myc tag. The predicted molecular weight is 39.9 kDa and the accession number is Q4R8Y9.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$5020 days20 days
10 μg$7920 days20 days
20 μg$12720 days20 days
50 μg$22520 days20 days
100 μg$35020 days20 days
200 μg$63320 days20 days
500 μg$1,39020 days20 days
1 mg$2,55020 days20 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis TPBG at 2 μg/mL can bind Anti-TPBG recombinant antibody, the EC50 is 1.672-2.181 ng/mL.
Description
May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. 5T4/TPBG Protein, Cynomolgus, Recombinant (His & Myc) is expressed in HEK293 mammalian cells with N-10xHis and C-Myc tag. The predicted molecular weight is 39.9 kDa and the accession number is Q4R8Y9.
Species
Cynomolgus
Expression System
HEK293 Cells
TagN-10xHis, C-Myc
Accession NumberQ4R8Y9
Amino AcidTSSASSSSSSAPFLASAASAQPPLPDQCPALCECSEAARTVKCVNRNLTEVPTDLPLYVRNLFLTGNQLAVLPAGAFARRPPLAELAALNLSGSRLDEVRGGAFEHLPSLRQLDLSHNPLAYLSPFAFSGSNASISAPSPLVELILNHIVPPDDKRQNRSFEGMVAAALVAGRALQGLHLLELASNHFLYLPRDVLAQLPSLRYLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHVRVFLDNNPWVCDCHMADMVTWLKQTGVVQGKDRLTCAFPEKMRNRVLLELNSADLDCDPILPPSLQTS
Construction35-355 aa
Protein Purity
> 95% as determined by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
ReconstitutionReconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsWnt-activated inhibitory factor 1 (WAIF1), Trophoblast glycoprotein, TPBG
Research Background
Chemical Properties
Molecular Weight39.9 kDa (predicted)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: 5T4/TPBG Protein, Cynomolgus, Recombinant (His & Myc) chemical structure | 5T4/TPBG Protein, Cynomolgus, Recombinant (His & Myc) molecular weight