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PVRIG Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 42.31 kDa and the accession number is Q6DKI7-1.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 5 μg | $167 | 7-10 days | 7-10 days | |
| 10 μg | $277 | 7-10 days | 7-10 days | |
| 20 μg | $462 | 7-10 days | 7-10 days | |
| 50 μg | $746 | 7-10 days | 7-10 days | |
| 100 μg | $1,230 | 7-10 days | 7-10 days |
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human Nectin-2 hFc at 2 μg/mL (100 μL/well) can bind Human PVRIG (hFc & AVI Tag), Biotinylated, the EC50 of Human PVRIG (hFc & AVI Tag), Biotinylated is 40-220 ng/mL. |
| Description | PVRIG Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 42.31 kDa and the accession number is Q6DKI7-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-hFc-Avi |
| Accession Number | Q6DKI7-1 |
| Synonyms | RMO1,RalGDS/AF-6,PREL-2,PREL2,LPD,C7orf15,ALS2CR9,ALS2CR18 |
| Construction | A DNA sequence encoding the human PVRIG (Q6DKI7-1)(Thr41-Leu172) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Thr 41 |
| Protein Purity | > 90 % as determined by SDS-PAGE. |
| Molecular Weight | 42.31 kDa (predicted); 58.43 kDa (reducing conditions) |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
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