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Anti-5T4/TPBG Antibody (2R764)

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Catalog No. TMAH-01207
Alias WAIF1, TPBG, M6P1, 5T4AG, 5T4

Anti-5T4/TPBG Antibody (2R764) is an antibody targeting 5T4/TPBG. Anti-5T4/TPBG Antibody (2R764) can be used in ELISA, WB, IHC.

Anti-5T4/TPBG Antibody (2R764)

Anti-5T4/TPBG Antibody (2R764)

😃Good
Catalog No. TMAH-01207Alias WAIF1, TPBG, M6P1, 5T4AG, 5T4
Anti-5T4/TPBG Antibody (2R764) is an antibody targeting 5T4/TPBG. Anti-5T4/TPBG Antibody (2R764) can be used in ELISA, WB, IHC.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$347 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IHC

Reactivity: Human, Mouse
AliasWAIF1, TPBG, M6P1, 5T4AG, 5T4
Ig Type
Rabbit IgG
Clone
2R764
Reactivity
Human, Mouse
Verified Activity
1. Western Blot
-Positive WB detected in: U-87 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate
-All lanes: 5T4 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 47 kDa
-Observed band size: 50, 80 kDa
2. IHC image of TMAH-01207 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Application
ELISA, WB, IHC
Recommended Dose
WB:1:500-1:5000; IHC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCell membrane; Single-pass type I membrane protein.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundMay function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human 5T4
Antigen Species
Human
Gene ID
7162
Uniprot ID
Biology Area
Cancer, Developmental biology, Immunology, Stem cells
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Sci Citations

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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