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Anti-LDLR Antibody (3N325)
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Catalog No. TMAY-03221
Alias low density lipoprotein receptor, LDLCQ2, LDL Receptor, LDL R, FHC, FH
Anti-LDLR Antibody (3N325) is a Rabbit antibody targeting LDLR. Anti-LDLR Antibody (3N325) can be used in ELISA,FCM,ICC/IF.
Anti-LDLR Antibody (3N325)
Anti-LDLR Antibody (3N325)
😃Good
Catalog No. TMAY-03221Alias low density lipoprotein receptor, LDLCQ2, LDL Receptor, LDL R, FHC, FH
Anti-LDLR Antibody (3N325) is a Rabbit antibody targeting LDLR. Anti-LDLR Antibody (3N325) can be used in ELISA,FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $292 | 7-10 days |
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Chemical Properties
| Description | Anti-LDLR Antibody (3N325) is a Rabbit antibody targeting LDLR. Anti-LDLR Antibody (3N325) can be used in ELISA,FCM,ICC/IF. |
| Alias | low density lipoprotein receptor, LDLCQ2, LDL Receptor, LDL R, FHC, FH |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 3N325 |
| Reactivity | Mouse |
| Verified Activity | 1. Immunofluorescence staining of Mouse LDLR in raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Mouse LDLR monoclonal antibody (1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) Goat Anti-rabbit IgG secondary antibody(green), countstained with DAPI (blue). Positive staining was localized to plasma membrane. 2. Flow cytometric analysis of Mouse LDLR expression on Raw264.7. Cells were stained with purified anti-Mouse LDLR, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | ELISA,FCM,ICC/IF |
| Recommended Dose | ELISA: 1:5000-1:10000; ICC-IF: 1:50-1:1000; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LDLR / LDL Receptor (rM LDLR; TMPY-01014; Met 1-Arg 790; NP_034830.2). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | LDL Receptor, also known as LDLR, is a mosaic protein that belongs to the Low-density lipoprotein receptor gene family. The low-density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDL Receptor consists of 840 amino acids (after removal of signal peptide) and mediates the endocytosis of cholesterol-rich LDL. Low-density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. LDL Receptor is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants. |
| Conjucates | Unconjugated |
| Others Formats | PerCP/FITC/PE |
| Antibody Types Available | 4 |
| Immunogen | Recombinant Mouse LDLR protein (TMPY-01014) |
| Antigen Species | Mouse |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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