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IL-33 Protein, Human, Recombinant

IL-33 Protein, Human, Recombinant
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IL-33 Protein, Human, Recombinant

Catalog No. TMPY-00861
IL-33 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18 kDa and the accession number is O95760-1.
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Pack SizePriceAvailabilityQuantity
5 μg$57In Stock
10 μg$917-10 days
20 μg$1477-10 days
50 μg$2777-10 days
100 μg$4477-10 days
200 μg$7637-10 days
500 μg$1,5407-10 days
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Biological Description

Biological Information
1. Measured by its binding ability in a functional ELISA. Immobilized human IL-33 at 10 μg/ml (100 μl/well) can bind human IL1RL1 with a linear range of 0.31-5 ng/ml._x000D_ 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 1-7 ng/mL.
Description
IL-33 Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 18 kDa and the accession number is O95760-1.
Species
Human
Expression System
E. coli
TagTag Free
Accession NumberO95760-1
Synonyms
IL1F11,C9orf26,NFEHEV,NF-HEV,DVS27,interleukin 33
Construction
The C-terminal segment of Human IL33 (NP_254274.1) (Ser 112-Thr 270) was expressed with an initial Met at the N-terminus.
Protein Purity
> 95 % as determined by SDS-PAGE
Molecular Weight18 kDa (predicted)
EndotoxinPlease contact us for more information.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial Venules), is a pro-inflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyer's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.

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