IL-33 Protein, Human, Recombinant

Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial Venules), is a pro-inflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyer's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.

Pack Size	Availability	Price/USD
5 μg	In stock	$ 57.00
10 μg	5 days	$ 91.00
20 μg	5 days	$ 147.00
50 μg	5 days	$ 277.00
100 μg	5 days	$ 447.00
200 μg	5 days	$ 763.00
500 μg	5 days	$ 1,540.00

Pack Size

Availability

Price/USD

Quantity

5 μg

In stock

$ 57.00

10 μg

5 days

$ 91.00

20 μg

5 days

$ 147.00

50 μg

5 days

$ 277.00

100 μg

5 days

$ 447.00

200 μg

5 days

$ 763.00

500 μg

5 days

$ 1,540.00

Biological Information

1. Measured by its binding ability in a functional ELISA. Immobilized human IL-33 at 10 μg/ml (100 μl/well) can bind human IL1RL1 with a linear range of 0.31-5 ng/ml. 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 1-7 ng/mL.

Description

Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	O95760-1
Synonyms	IL1F11, C9orf26, NFEHEV, NF-HEV, DVS27, interleukin 33
Construction	A DNA sequence encoding the C-terminal segment of human IL33 (NP_254274.1) (Ser 112-Thr 270) was expressed with an initial Met at the N-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	Approxiamtely 18 kDa

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial Venules), is a pro-inflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyer's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.