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Anti-Aminopeptidase N/CD13 Antibody (8T921)
😃Good
Catalog No. TMAY-00184
Alias P150, Cd13, AP-N, Apn, AP-M, alanyl (membrane) aminopeptidase
Anti-Aminopeptidase N/CD13 Antibody (8T921) is a Rabbit antibody targeting Aminopeptidase N/CD13. Anti-Aminopeptidase N/CD13 Antibody (8T921) can be used in WB,ELISA,IHC-P.
Anti-Aminopeptidase N/CD13 Antibody (8T921)
Anti-Aminopeptidase N/CD13 Antibody (8T921)
😃Good
Catalog No. TMAY-00184Alias P150, Cd13, AP-N, Apn, AP-M, alanyl (membrane) aminopeptidase
Anti-Aminopeptidase N/CD13 Antibody (8T921) is a Rabbit antibody targeting Aminopeptidase N/CD13. Anti-Aminopeptidase N/CD13 Antibody (8T921) can be used in WB,ELISA,IHC-P.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Chemical Properties
| Description | Anti-Aminopeptidase N/CD13 Antibody (8T921) is a Rabbit antibody targeting Aminopeptidase N/CD13. Anti-Aminopeptidase N/CD13 Antibody (8T921) can be used in WB,ELISA,IHC-P. |
| Alias | P150, Cd13, AP-N, Apn, AP-M, alanyl (membrane) aminopeptidase |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 8T921 |
| Reactivity | Human |
| Verified Activity | 1. Immunochemical staining of human ANPEP in human renal carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). 2. Immunochemical staining of human ANPEP in human liver with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). 3. Anti-ANPEP rabbit monoclonal antibody at 1:500 dilution. -Lane A: THP1 Whole Cell Lysate. -Lane B: U937 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:110 kDa. -Observed band size:140 kDa |
| Application | WB,ELISA,IHC-P |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IHC-P: 1:100-1:500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human CD13 / ANPEP (rh CD13 / ANPEP; TMPY-00665; NP_001141.2; Lys 69-Lys 967, 603 Met/Ile). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Aminopeptidase N (ANPEP or APN), also known as CD13, is a cell-surface metalloprotease located in the small-intestinal and renal microvillar membrane, as well as other plasma membranes. It belongs to the peptidase M1 family. CD13 plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases and is involved in the metabolism of regulatory peptides by diverse cell types. CD13/APN is a potent regulator of angiogenesis which is essential for tumor invasion and metastasis, and its transcription in activated endothelial cells is induced by angiogenic growth factors via the RAS/MAPK pathway. In addition, this enzyme has been shown to participate in antigen processing and presentation, and accordingly, defects in this gene appear to be a cause of various types of leukemia or lymphoma and carcinomas. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human CD13 / ANPEP protein (TMPY-00665) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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