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Anti-Phospho-ACAP1 (Ser554) Polyclonal Antibody
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Catalog No. TMAC-00052
Alias KIAA0050, Cnt-b1, CENTB1, Centaurin-beta-1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1, ACAP 1
Anti-Phospho-ACAP1 (Ser554) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ACAP1 (Ser554). Anti-Phospho-ACAP1 (Ser554) Polyclonal Antibody can be used in WB,IHC-P,IF(paraffin section),ELISA.
Anti-Phospho-ACAP1 (Ser554) Polyclonal Antibody
Anti-Phospho-ACAP1 (Ser554) Polyclonal Antibody
😃Good
Catalog No. TMAC-00052Alias KIAA0050, Cnt-b1, CENTB1, Centaurin-beta-1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1, ACAP 1
Anti-Phospho-ACAP1 (Ser554) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ACAP1 (Ser554). Anti-Phospho-ACAP1 (Ser554) Polyclonal Antibody can be used in WB,IHC-P,IF(paraffin section),ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $217 | 7-10 days | |
| 100 μL | $317 | 7-10 days |
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| Description | Anti-Phospho-ACAP1 (Ser554) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ACAP1 (Ser554). Anti-Phospho-ACAP1 (Ser554) Polyclonal Antibody can be used in WB,IHC-P,IF(paraffin section),ELISA. |
| Alias | KIAA0050, Cnt-b1, CENTB1, Centaurin-beta-1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1, ACAP 1 |
| Ig Type | IgG |
| Reactivity | Human |
| Application | |
| Recommended Dose | IHC-P: 1:100-300; ELISA: 1:40000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Appearance | Liquid |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research Background | domain:PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes.,enzyme regulation:GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.,function:GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface.,miscellaneous:Cells overexpressing ACAP1 show an accumulation of ITGB1 in recycling endosomes and inhibition of stimulation-dependent cell migration. Cells with reduced levels of ACAP1 or AKT1 and AKT2 show inhibition of stimulation-dependent cell migration. Cells overexpressing ACAP1 and PIP5K1C show formation of tubular structures derived from endosomal membranes.,PTM:Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.,similarity:Contains 1 Arf-GAP domain.,similarity:Contains 1 BAR domain.,similarity:Contains 1 PH domain.,similarity:Contains 3 ANK repeats.,subunit:Interacts with GTP-bound ARF6. Interacts with third cytoplasmic loop of SLC2A4/GLUT4. Interacts with CLTC. Interacts with GULP1. Forms a complex with GDP-bound ARF6 and GULP1.,tissue specificity:Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas., |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | A synthesized phosphopeptide: human Centaurin-beta1 around the phosphorylation site of Ser554. AA range:520-569 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 81 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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