Antibody Type: Mouse Monoclonal

Application: ELISA, WB, IHC, IF, FCM

Reactivity: Human

IgG1

9E461

Human

1. Western Blot
-Positive WB detected in: U251 whole cell lysate, SH-SY5Y whole cell lysate
-All lanes: NES antibody at 3µg/ml
-Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution
-Predicted band size: 178 kDa
-Observed band size: 260 kDa
2. Immunohistochemistry of paraffin-embedded human tonsil tissue using TMAH-00811 at dilution of 1:100
3. Immunohistochemistry of paraffin-embedded human kidney tissue using TMAH-00811 at dilution of 1:100
4. Immunohistochemistry of paraffin-embedded human melanoma using TMAH-00811 at dilution of 1:100
5. Immunofluorescent analysis of Hela cells using TMAH-00811 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
6. Immunofluorescent analysis of PC-3 cells using TMAH-00811 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
7. Immunofluorescent analysis of U251 cells using TMAH-00811 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
8. Overlay histogram showing U251 cells stained with TMAH-00811 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*10^6 cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG1 (10µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
9. Overlay histogram showing Hela cells stained with TMAH-00811 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*10^6 cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG1 (10µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IHC, IF, FCM

Monoclonal

Unconjugated

Recombinant Protein: Human Nestin Protein

Human

10763

Neuroscience