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Anti-JUN Antibody (1D644)

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Catalog No. TMAH-00657

Anti-JUN Antibody (1D644) is an antibody targeting JUN. Anti-JUN Antibody (1D644) can be used in ELISA, WB, IHC.

Anti-JUN Antibody (1D644)

Anti-JUN Antibody (1D644)

😃Good
Catalog No. TMAH-00657
Anti-JUN Antibody (1D644) is an antibody targeting JUN. Anti-JUN Antibody (1D644) can be used in ELISA, WB, IHC.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$348 7-10 days
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Instruction Manual of Detection Antibody
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Monoclonal

Application: ELISA, WB, IHC

Reactivity: Human
Ig Type
Rabbit IgG
Clone
1D644
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: 293 whole cell lysate
-All lanes: JUN antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 36 kDa
-Observed band size: 43 kDa
2. IHC image of TMAH-00657 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Application
ELISA, WB, IHC
Recommended Dose
WB:1:500-1:5000; IHC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationNucleus.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundTranscription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. Binds to the USP28 promoter in colorectal cancer (CRC) cells.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human c-Jun
Antigen Species
Human
Gene ID
3725
Uniprot ID
Biology Area
Epigenetics and Nuclear Signaling, Cancer, Immunology, Signal transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Sci Citations

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

V-jun avian sarcoma virus 17 oncogene homologTranscription factor JunTranscription factor AP-1 subunit JunProto-oncogene c-Junp39p 39JUNAP1AP 1Activator protein 1 (AP1)
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