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NKG2D/CD314 Protein, Human, Recombinant (His & Avi), Biotinylated

TargetMol | SPR
Catalog No. TMPK-00106 Copy Product Info
NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells. NKG2D/CD314 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 19.0 kDa and the accession number is P26718.

NKG2D/CD314 Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPK-00106
Copy Product Info
TargetMol | SPR

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells. NKG2D/CD314 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 19.0 kDa and the accession number is P26718.

NKG2D/CD314 Protein, Human, Recombinant (His & Avi), Biotinylated
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$917-10 days7-10 days
10 μg$1487-10 days7-10 days
20 μg$2457-10 days7-10 days
50 μg$4837-10 days7-10 days
100 μg$8137-10 days7-10 days
200 μg$1,4707-10 days7-10 days
500 μg$3,2507-10 days7-10 days
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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)
For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

Product Information

Biological Activity
Immobilized Anti-NKG2D Antibody at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human NKG2D, His Tag with the EC50 of 0.39μg/ml determined by ELISA.
Description
NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells. NKG2D/CD314 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 19.0 kDa and the accession number is P26718.
Species
Human
Expression System
HEK293 Cells
TagN-His-Avi
Accession NumberP26718
Synonyms
NKG2-D,NKG2D,KLR,D12S2489E,CD314
Construction
Phe78-Val216
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight19.0 kDa (predicted). Due to glycosylation, the protein migrates to 36-40 kDa based on Tris-Bis PAGE result.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background
NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.

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