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Anti-MCM9 Antibody (5A998)
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Catalog No. TMAH-00742
Alias Mini-chromosome maintenance deficient domain-containing protein 1, Minichromosome maintenance 9, MCMDC1DNA helicase MCM9, MCM 9, hMCM9, EC 3.6.4.12, C6orf61
Anti-MCM9 Antibody (5A998) is a Mouse antibody targeting MCM9. Anti-MCM9 Antibody (5A998) can be used in ELISA, WB, IHC.
Anti-MCM9 Antibody (5A998)
Anti-MCM9 Antibody (5A998)
😃Good
Catalog No. TMAH-00742Alias Mini-chromosome maintenance deficient domain-containing protein 1, Minichromosome maintenance 9, MCMDC1DNA helicase MCM9, MCM 9, hMCM9, EC 3.6.4.12, C6orf61
Anti-MCM9 Antibody (5A998) is a Mouse antibody targeting MCM9. Anti-MCM9 Antibody (5A998) can be used in ELISA, WB, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ELISA, WB, IHC Reactivity: Human |
| Alias | Mini-chromosome maintenance deficient domain-containing protein 1, Minichromosome maintenance 9, MCMDC1DNA helicase MCM9, MCM 9, hMCM9, EC 3.6.4.12, C6orf61 |
| Ig Type | IgG1 |
| Clone | 5A998 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: SY-5Y whole cell lysate, Hela whole cell lysate,U87 whole cell lysate -All lanes: MCM9 antibody at 1:1000 -Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution -Predicted band size: 128, 73, 44, 85 kDa -Observed band size: 128 kDa -Exposure time：5min 2. IHC image of TMAH-00742 diluted at 1:100 and staining in paraffin-embedded human testes tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Application | ELISA, WB, IHC |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Nucleus. Chromosome. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Purity | >95% |
| Research Background | Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MRN complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand. In addition, recruits MLH1, a component of the MMR complex, to chromatin. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. Probably by regulating HR, plays a key role during gametogenesis. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human DNA helicase MCM9 Protein (1-391AA) |
| Antigen Species | Human |
| Gene ID | 254394 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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