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Anti-STAT1 Antibody (3B679)

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Catalog No. TMAH-01136
Alias STAT91, signal transducer and activator of transcription 1, 91kDa, ISGF-3, IMD31C, IMD31B, IMD31A, CANDF7

Anti-STAT1 Antibody (3B679) is an antibody targeting STAT1. Anti-STAT1 Antibody (3B679) can be used in ELISA, IHC, IF, FCM.

Anti-STAT1 Antibody (3B679)

Anti-STAT1 Antibody (3B679)

😃Good
Catalog No. TMAH-01136Alias STAT91, signal transducer and activator of transcription 1, 91kDa, ISGF-3, IMD31C, IMD31B, IMD31A, CANDF7
Anti-STAT1 Antibody (3B679) is an antibody targeting STAT1. Anti-STAT1 Antibody (3B679) can be used in ELISA, IHC, IF, FCM.
Pack SizePriceAvailabilityQuantity
50 μL$209 7-10 days
100 μL$347 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, IHC, IF, FCM

Reactivity: Human
AliasSTAT91, signal transducer and activator of transcription 1, 91kDa, ISGF-3, IMD31C, IMD31B, IMD31A, CANDF7
Ig Type
Rabbit IgG
Clone
3B679
Reactivity
Human
Verified Activity
1. IHC image of TMAH-01136 diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.40% DAB.
2. Immunofluorescence staining of Hela with TMAH-01136 at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 508-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
3. Overlay Peak curve showing HepG2 cells stained with TMAH-01136 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Application
ELISA, IHC, IF, FCM
Recommended Dose
IHC:1:50-1:200; IF:1:50-1:200; FCM:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCytoplasm. Nucleus.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundSignal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human STAT1
Antigen Species
Human
Gene ID
6772
Uniprot ID
Biology Area
Epigenetics and Nuclear Signaling, Cancer, Signal transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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