PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | 5 days | $ 347.00 | |
500 μg | 5 days | $ 1,400.00 | |
1 mg | 5 days | $ 2,330.00 |
Description | PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. |
Species | Human |
Expression System | HEK293 |
Tag | C-His-Avi |
Accession Number | P09619 |
Synonyms | PDGFR1, CD140B, PDGFR-2, PDGF R β/CD140b, PDGF R beta, PDGFRB, PDGFR-β, JTK12, PDGFR, PDGF R β |
Construction | Recombinant Human PDGF R beta/CD140b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu33-Phe530.[Accession |P09619] |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | The protein has a predicted MW of 59 kDa. Due to glycosylation, the protein migrates to 78-115 kDa based on Tris-Bis PAGE result. |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA,PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. PDGF R plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells. Plays a role in the migration of vascular smooth muscle cells and the formation of neointima at vascular injury sites. |
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Please read the User Guide of Recombinant Proteins for more specific information.
PDGF R beta/CD140b Protein, Human, Recombinant (His & Avi) PDGFR1 JTK 12 CD140B PDGFR2 PDGFR-1 PDGFR-2 PDGFR-beta JTK-12 PDGF R β/CD140b PDGF R beta PDGFRB PDGFR-β PDGF R b JTK12 PDGFR PDGF R β PDGFR-b PDGFR 2 PDGFR 1 recombinant recombinant-proteins proteins protein