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Synonyms: Harvey rat sarcoma viral oncogene homolog

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $79 | 7-10 days | 7-10 days | |
| 10 μg | $129 | 7-10 days | 7-10 days | |
| 20 μg | $208 | 7-10 days | 7-10 days | |
| 50 μg | $419 | 7-10 days | 7-10 days | |
| 100 μg | $812 | 7-10 days | 7-10 days |
| Bioactivity | 1. Immobilized Recombinant Influenza A H1N1(A/California/07/2009)HA trimer Protein(His Tag) at 5μg/mL (100μL/well) can bind Recombinant Human Galectin-1 / LGALS1 Protein , the EC50 is 120-360 ng/mL.
2. Immobilized Recombinant Influenza A H1N1(A/California/07/2009)HA trimer Protein(His Tag) at 1μg/mL (100μL/well) can bind antibody targets the HA stem region of H1N1, the EC50 is 4.5-15 ng/mL (QC tested). |
| Description | Influenza A H1N1 (A/California/07/2009) Hemagglutinin/HA trimer Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 61.94 kDa. |
| Species | H1N1 |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Construction | A DNA sequence encoding the Influenza A virus ((A/California/07/2009) (H1N1)) hemagglutinin (translated amino acids of ACP41953.1), termed as HA, was expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus. Predicted N terminal: Asp 18 |
| Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 95% as determined by SEC-MALS. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | Harvey rat sarcoma viral oncogene homolog |
| Research Background |
| Molecular Weight | 61.94 kDa (predicted); 75 kDa (reducing conditions) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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