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Influenza A H1N1 (A/California/07/2009) Hemagglutinin/HA trimer Protein (His)

(Synonyms: Harvey rat sarcoma viral oncogene homolog) Copy Product Info

Synonyms: Harvey rat sarcoma viral oncogene homolog

Catalog No. TMPY-06332 Copy Product Info
Influenza A H1N1 (A/California/07/2009) Hemagglutinin/HA trimer Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 61.94 kDa.
Pack SizePriceUSA StockGlobal StockQuantity
5 μg$797-10 days7-10 days
10 μg$1297-10 days7-10 days
20 μg$2087-10 days7-10 days
50 μg$4197-10 days7-10 days
100 μg$8127-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
1. Immobilized Recombinant Influenza A H1N1(A/California/07/2009)HA trimer Protein(His Tag)  at 5μg/mL (100μL/well) can bind Recombinant Human Galectin-1 / LGALS1 Protein , the EC50 is 120-360 ng/mL. 2. Immobilized Recombinant Influenza A H1N1(A/California/07/2009)HA trimer Protein(His Tag) at 1μg/mL (100μL/well) can bind antibody targets the HA stem region of H1N1, the EC50 is 4.5-15 ng/mL (QC tested).
Description
Influenza A H1N1 (A/California/07/2009) Hemagglutinin/HA trimer Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 61.94 kDa.
Species
H1N1
Expression System
HEK293 Cells
TagC-His
ConstructionA DNA sequence encoding the Influenza A virus ((A/California/07/2009) (H1N1)) hemagglutinin (translated amino acids of ACP41953.1), termed as HA, was expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus. Predicted N terminal: Asp 18
Protein Purity
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 95% as determined by SEC-MALS.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsHarvey rat sarcoma viral oncogene homolog
Research Background
Chemical Properties
Molecular Weight61.94 kDa (predicted); 75 kDa (reducing conditions)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Influenza A H1N1 (A/California/07/2009) Hemagglutinin/HA trimer Protein (His) chemical structure | Influenza A H1N1 (A/California/07/2009) Hemagglutinin/HA trimer Protein (His) molecular weight