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CD40 Ligand Trimer Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His-Avi & Flag)
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Catalog No. TMPK-01650 Copy Product Info
CD40 Ligand Trimer Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His-Avi & Flag) is expressed in HEK293 Cells. The accession number is P29965(Human)/G7Q1T4(Cynomolgus)/P63304(Rhesus macaque).
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 56.2 ng/ml determined by ELISA. |
| Description | CD40 Ligand Trimer Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His-Avi & Flag) is expressed in HEK293 Cells. The accession number is P29965(Human)/G7Q1T4(Cynomolgus)/P63304(Rhesus macaque). |
| Species | Human/Cynomolgus/Rhesus macaque |
| Expression System | HEK293 Cells |
| Tag | N-His-Avi, N-Flag |
| Accession Number | P29965 (Human)/G7Q1T4 (Cynomolgus)/P63304 (Rhesus macaque) |
| Construction | Met113-Leu261 trimer design |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Research Background |
| Molecular Weight | 54.51 kDa (Predicted); 55-70 kDa (Reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: CD40 Ligand Trimer Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His-Avi & Flag) chemical structure | CD40 Ligand Trimer Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His-Avi & Flag) molecular weight
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