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CD40 Ligand Trimer Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His-Avi & Flag)

(Synonyms: TRAP, TNFSF5IMD3, TNFSF5, T-BAM, IMD3, IGM, HIGM1, gp39, CD40LIGM, CD40LG, CD40L, CD40 Ligand, CD154) Copy Product Info

Synonyms: TRAP, TNFSF5IMD3, TNFSF5, T-BAM, IMD3, IGM, HIGM1, gp39, CD40LIGM, CD40LG, CD40L, CD40 Ligand, CD154

Catalog No. TMPK-01650 Copy Product Info
CD40 Ligand Trimer Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His-Avi & Flag) is expressed in HEK293 Cells. The accession number is P29965(Human)/G7Q1T4(Cynomolgus)/P63304(Rhesus macaque).
CD40 Ligand Trimer Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His-Avi & Flag)
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Pack SizePriceUSA StockGlobal StockQuantity
5 µg$1087-10 days7-10 days
10 µg$1697-10 days7-10 days
20 µg$2677-10 days7-10 days
50 µg$4887-10 days7-10 days
100 µg$7767-10 days7-10 days
200 µg$1,2307-10 days7-10 days
500 µg$2,2907-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
Immobilized Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 56.2 ng/ml determined by ELISA.
Description
CD40 Ligand Trimer Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His-Avi & Flag) is expressed in HEK293 Cells. The accession number is P29965(Human)/G7Q1T4(Cynomolgus)/P63304(Rhesus macaque).
Species
Human/Cynomolgus/Rhesus macaque
Expression System
HEK293 Cells
TagN-His-Avi, N-Flag
Accession NumberP29965(Human)/G7Q1T4(Cynomolgus)/P63304(Rhesus macaque)
ConstructionMet113-Leu261 trimer design
Protein Purity
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin< 1 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsTRAP, TNFSF5IMD3, TNFSF5, T-BAM, IMD3, IGM, HIGM1, gp39, CD40LIGM, CD40LG, CD40L, CD40 Ligand, CD154
Research Background
CD40 ligand or CD40L, also called CD154,  is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
Chemical Properties
Molecular Weight54.51 kDa (Predicted); 55-70 kDa (Reducing condition, due to glycosylation)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: CD40 Ligand Trimer Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His-Avi & Flag) chemical structure | CD40 Ligand Trimer Protein, Human/Cynomolgus/Rhesus macaque, Recombinant (His-Avi & Flag) molecular weight