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Anti-FIS1 Antibody (9Z765) is an antibody targeting FIS1. Anti-FIS1 Antibody (9Z765) can be used in ELISA,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $207 | 7-10 days | 7-10 days | |
| 100 μL | $349 | 7-10 days | 7-10 days |
| Description | Anti-FIS1 Antibody (9Z765) is an antibody targeting FIS1. Anti-FIS1 Antibody (9Z765) can be used in ELISA,IHC,WB. |
| Synonyms | TTC11, TPR Repeat Protein 11, Tetratricopeptide Repeat Protein 11, Mitochondrial Fission 1 Protein, hFis1, FIS1 Homolog, FIS1, CGI-135 |
| Ig Type | Rabbit IgG |
| Clone | 9Z765 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: HepG2 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, U251 whole cell lysate, A549 whole cell lysate -All lanes: FIS1 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 17 kDa -Observed band size: 17-22 kDa 2. IHC image of TMAH-00438 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00438 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
| Application | |
| Recommended Dose | WB:1:500-1:2000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Mitochondrion outer membrane; Single-pass membrane protein. Peroxisome membrane; Single-pass membrane protein. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human FIS1 |
| Antigen Species | Human |
| Gene Name | FIS1 |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cancer, Cell biology, Metabolism |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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