Antibody Type: Recombinant Mouse Monoclonal

Application: ELISA, WB, IHC, IF

Reactivity: Human

mFc2a

9G387

Human

1. Western Blot
-Positive WB detected in: A549 whole cell lysatee
-All lanes: SYP antibody at 1:500
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 34 kDa
-Observed band size: 34 kDa
2. IHC image of TMAH-01153 diluted at 1:200 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
3. Immunofluorescence staining of Hela cell with TMAH-01153 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

ELISA, WB, IHC, IF

WB:1:500-1:2000; IHC:1:50-1:200; IF:1:50-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human SYP Protein

Human

6855

Neuroscience