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B7-H3 Protein, Human, Recombinant (aa 29-245, His)
(Synonyms: PSEC0249, PRO352, CD276, B7-H3, B7H3, B7 homolog 3, UNQ309) Copy Product InfoSynonyms: PSEC0249, PRO352, CD276, B7-H3, B7H3, B7 homolog 3, UNQ309
Catalog No. TMPK-01018 Copy Product Info
B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. B7-H3 Protein, Human, Recombinant (aa 29-245, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 24.7 kDa and the accession number is Q5ZPR3-2.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $46 | 7-10 days | 7-10 days | |
| 10 µg | $72 | 7-10 days | 7-10 days | |
| 20 µg | $115 | 7-10 days | 7-10 days | |
| 50 µg | $222 | 7-10 days | 7-10 days | |
| 100 µg | $371 | 7-10 days | 7-10 days | |
| 200 µg | $673 | 7-10 days | 7-10 days | |
| 500 µg | $1,490 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Human B7-H3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 29.4ng/ml determined by ELISA. |
| Description | B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. B7-H3 Protein, Human, Recombinant (aa 29-245, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 24.7 kDa and the accession number is Q5ZPR3-2. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | Q5ZPR3-2 |
| Construction | Leu29-Pro245 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | PSEC0249, PRO352, CD276, B7-H3, B7H3, B7 homolog 3, UNQ309 |
| Research Background | B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction. |
| Molecular Weight | 24.7 kDa (predicted). Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: B7-H3 Protein, Human, Recombinant (aa 29-245, His) chemical structure | B7-H3 Protein, Human, Recombinant (aa 29-245, His) molecular weight
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