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Anti-SPARCL1 Antibody (5B937)

😃Good
Catalog No. TMAY-00164
Alias SPARC like 1, Sc1, mast9, hevin, Ecm2

Anti-SPARCL1 Antibody (5B937) is a Mouse antibody targeting SPARCL1. Anti-SPARCL1 Antibody (5B937) can be used in ELISA,FCM,ICC/IF.

Anti-SPARCL1 Antibody (5B937)

Anti-SPARCL1 Antibody (5B937)

😃Good
Catalog No. TMAY-00164Alias SPARC like 1, Sc1, mast9, hevin, Ecm2
Anti-SPARCL1 Antibody (5B937) is a Mouse antibody targeting SPARCL1. Anti-SPARCL1 Antibody (5B937) can be used in ELISA,FCM,ICC/IF.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Anti-SPARCL1 Antibody (5B937) is a Mouse antibody targeting SPARCL1. Anti-SPARCL1 Antibody (5B937) can be used in ELISA,FCM,ICC/IF.
AliasSPARC like 1, Sc1, mast9, hevin, Ecm2
Ig Type
Monoclonal Mouse IgG1
Clone
5B937
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human SPARCL1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human SPARCL1 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoplasm.

2. Flow cytometric analysis of Human SPARCL1 expression on HL60 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human SPARCL1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
ELISA,FCM,ICC/IF
Recommended Dose
ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SPARCL1 (rhSPARCL1; NP_004675.3Met 1-Phe 664). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundSPARC-like protein 1 (SPARCL1; also known as SC1, high endothelial venule protein, or hevin) is an extracellular matrix-associated, secreted glycoprotein belonging to the secreted protein acidic and rich in cysteine (SPARC) family of matricellular proteins. It contains three conserved structural domains that are implicated in the regulation of cell adhesion, migration, and proliferation. SPARCL1 is expressed during embryogenesis and tissue remodeling and is especially prominent in brain and vasculature. Its down-regulation in a number of cancers and the possibility of its functional compensation by SPARC has led to recent interest in hevin as a tumor suppressor and regulator of angiogenesis. SPARCL1 has antiadhesive properties, and loss of SPARCL1 expression is associated with increased proliferative activity and cell cycle progression. It is suggested that it may influence multiple cellular processes during distinct stages of brain development and function. Besides, SPARCL1 can influence the function of astroglial cells in the developing and mature central nervous system (CNS).
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
FITC/PE
Antibody Types Available
3
Antigen Details
Immunogen
Recombinant Human SPARCL1 protein
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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