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Carbonic Anhydrase 9 Protein, Human, Recombinant (hFc & Avi), Biotinylated
(Synonyms: MN, Carbonic Anhydrase IX, CAIX) Copy Product InfoSynonyms: MN, Carbonic Anhydrase IX, CAIX
Catalog No. TMPY-06939 Copy Product Info
Carbonic Anhydrase 9 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 69.51 kDa and the accession number is NP_001207.2.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $215 | 7-10 days | 7-10 days | |
| 10 μg | $359 | 7-10 days | 7-10 days | |
| 20 μg | $600 | 7-10 days | 7-10 days | |
| 50 μg | $939 | 7-10 days | 7-10 days | |
| 100 μg | $1,580 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1.Immobilized Anti-CA9 (Girentuximab biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human Carbonic Anhydrase IX Protein (Fc & Avi Tag),Biotinylated (Cat#TMPY-06939), the EC50 is 0.8-3 ng/mL(QC tested). 2.Immobilized Recombinant Human Carbonic Anhydrase IX Protein (Fc & Avi Tag), Biotinylated (Cat#TMPY-06939) at 1 μg/mL (100 μL/well) on Streptavidin precoated (5 μg/mL, 100 μL/well) plate can bind anti-CA9 (Girentuximab biosimilar), the EC50 is 4.3 ng/mL (Routinely tested). |
| Description | Carbonic Anhydrase 9 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 69.51 kDa and the accession number is NP_001207.2. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-hFc-Avi |
| Accession Number | NP_001207.2 |
| Construction | A DNA sequence encoding the Human CA9 (NP_001207.2)(Met1-Asp414) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Gln 38 |
| Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | MN, Carbonic Anhydrase IX, CAIX |
| Research Background |
| Molecular Weight | 69.51 kDa (predicted); 82.1 kDa (reducing conditions) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: Carbonic Anhydrase 9 Protein, Human, Recombinant (hFc & Avi), Biotinylated chemical structure | Carbonic Anhydrase 9 Protein, Human, Recombinant (hFc & Avi), Biotinylated molecular weight
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