Anti-Peripherin Polyclonal Antibody is a Rabbit antibody targeting Peripherin. Anti-Peripherin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

IgG

Human,Mouse,Rat

1. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Spinal cord tissue lysates
Primary: Anti-Peripherin (TMAB-10193) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 58 kDa
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Peripherin) Polyclonal Antibody, Unconjugated (TMAB-10193) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Peripherin) Polyclonal Antibody, Unconjugated (TMAB-10193) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Peripherin) Polyclonal Antibody, Unconjugated (TMAB-10193) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Peripherin) Polyclonal Antibody, Unconjugated (TMAB-10193) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Peripherin) Polyclonal Antibody, Unconjugated (TMAB-10193) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Peripherin) Polyclonal Antibody, Unconjugated (TMAB-10193) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
8. Measured by its binding ability in a indirect ELISA. Immobilized Human Peripherin protein, His Tag at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Human Peripherin Antibody, the EC50 is 124.3 ng/mL.

WB: 1:500-2000; IHC-P: 1:400-800; IHC-Fr: 1:400-800; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

Recombinant Protein: human Peripherin protein

Human

PRPH

Peripherin

Class-III neuronal intermediate filament protein.