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Anti-CYC1 Antibody (5W832)
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Catalog No. TMAH-00325
Alias Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, mitochondrial, heme protein, EC:7.1.1.8, Cytochrome c-1, Cytochrome c1, Cytochrome b-c1 complex subunit 4, Complex III subunit IV, Complex III subunit 4
Anti-CYC1 Antibody (5W832) is a Mouse antibody targeting CYC1. Anti-CYC1 Antibody (5W832) can be used in ELISA, WB, FC.
Anti-CYC1 Antibody (5W832)
Anti-CYC1 Antibody (5W832)
😃Good
Catalog No. TMAH-00325Alias Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, mitochondrial, heme protein, EC:7.1.1.8, Cytochrome c-1, Cytochrome c1, Cytochrome b-c1 complex subunit 4, Complex III subunit IV, Complex III subunit 4
Anti-CYC1 Antibody (5W832) is a Mouse antibody targeting CYC1. Anti-CYC1 Antibody (5W832) can be used in ELISA, WB, FC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ELISA, WB, FC Reactivity: Human, Mouse |
| Alias | Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, mitochondrial, heme protein, EC:7.1.1.8, Cytochrome c-1, Cytochrome c1, Cytochrome b-c1 complex subunit 4, Complex III subunit IV, Complex III subunit 4 |
| Ig Type | IgG1 |
| Clone | 5W832 |
| Reactivity | Human, Mouse |
| Verified Activity | 1. Western Blot -Positive WB detected in: CYC1 antibody at 1:1000 -Lane 1: Hela whole cell lysate -Lane 2: HepG2 whole cell lysate -Lane 3: MCF-7 whole cell lysate -Lane 4: K562 whole cell lysate -Lane 5: U251 whole cell lysate -Lane 6: A549 whole cell lysate -Lane 7: NIH/3T3 whole cell lysate -Lane 8: PC-3 whole cell lysate -Secondary: Goat polyclonal to Mouse IgG at 1/20000 dilution -Predicted band size: 35 KDa -Observed band size: 35 KDa -Exposure time: 5min 2. Overlay Peak curve showing HepG2 cells stained with TMAH-00325 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, WB, FC |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Mitochondrion inner membrane; Single-pass membrane protein. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Purity | >95% |
| Research Background | Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. Cytochrome c1 is a catalytic core subunit containing a c-type heme. It transfers electrons from the [2Fe-2S] iron-sulfur cluster of the Rieske protein to cytochrome c. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human Cytochrome c1 Protein (85-325AA) |
| Antigen Species | Human |
| Gene ID | 1537 |
| Uniprot ID | |
| Biology Area | Tags & Cell Markers |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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