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VEGF165 Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-00823
Synonyms: RP1-261G23.1, MVCD1, MGC70609, VEGFA, VEGFMGC70609, VPF, VEGF-165, VEGF, VAS

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF165 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 22.2 kDa and the accession number is P15692-4.

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VEGF165 Protein, Human, Recombinant (His & Avi)
Pack Size Availability Price/USD Quantity
100 μg In stock $ 465.00
500 μg 5 days $ 1,850.00
1 mg 5 days $ 3,100.00
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Biological Description
Technical Params
Product Properties
References and Literature
Biological Information Immobilized Human VEGF165 at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA.
Description Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF165 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 22.2 kDa and the accession number is P15692-4.
Species Human
Expression System HEK293 Cells
Tag C-His-Avi
Accession Number P15692-4
Synonyms RP1-261G23.1, MVCD1, MGC70609, VEGFA, VEGFMGC70609, VPF, VEGF-165, VEGF, VAS
Construction Ala27-Arg191
Protein Purity > 95% as determined by Tris-Bis PAGE
Molecular Weight 22.2 kDa (predicted). Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Endotoxin < 1 EU/μg by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

Research Background Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.

References and Literature

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Keywords

VEGF165 Protein, Human, Recombinant (His & Avi) MVCD 1 VEGFMGC-70609 RP1-261G23.1 MVCD-1 MVCD1 MGC70609 VEGFA VEGF 165 VEGFMGC70609 MGC 70609 VEGF165 VPF VEGF-165 MGC-70609 VEGF VAS VEGFMGC 70609 recombinant recombinant-proteins proteins protein

 

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