Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | In stock | $ 465.00 | |
500 μg | 5 days | $ 1,850.00 | |
1 mg | 5 days | $ 3,100.00 |
Biological Information | Immobilized Human VEGF165 at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA. |
Description | Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. |
Species | Human |
Expression System | HEK293 |
Tag | C-His-Avi |
Accession Number | P15692-4 |
Synonyms | RP1-261G23.1, MVCD1, MGC70609, VEGFA, VEGFMGC70609, VPF, VEGF-165, VEGF, VAS |
Construction | Recombinant Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala27-Arg191.[Accession |P15692-4] |
Protein Purity | > 95% as determined by Tris-Bis PAGE |
Molecular Weight | The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. |
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Please read the User Guide of Recombinant Proteins for more specific information.
VEGF165 Protein, Human, Recombinant (His & Avi) MVCD 1 VEGFMGC-70609 RP1-261G23.1 MVCD-1 MVCD1 MGC70609 VEGFA VEGF 165 VEGFMGC70609 MGC 70609 VEGF165 VPF VEGF-165 MGC-70609 VEGF VAS VEGFMGC 70609 recombinant recombinant-proteins proteins protein