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Anti-GRB2 Antibody (9L258)
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Catalog No. TMAH-00511
Alias SH2/SH3 Adapter GRB2, Protein Ash, Growth Factor Receptor-Bound Protein 2, GRB2, ASH, Adapter Protein GRB2
Anti-GRB2 Antibody (9L258) is an antibody targeting GRB2. Anti-GRB2 Antibody (9L258) can be used in ELISA, WB, IHC.
Anti-GRB2 Antibody (9L258)
Anti-GRB2 Antibody (9L258)
😃Good
Catalog No. TMAH-00511Alias SH2/SH3 Adapter GRB2, Protein Ash, Growth Factor Receptor-Bound Protein 2, GRB2, ASH, Adapter Protein GRB2
Anti-GRB2 Antibody (9L258) is an antibody targeting GRB2. Anti-GRB2 Antibody (9L258) can be used in ELISA, WB, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC Reactivity: Human, Mouse, Rat |
| Alias | SH2/SH3 Adapter GRB2, Protein Ash, Growth Factor Receptor-Bound Protein 2, GRB2, ASH, Adapter Protein GRB2 |
| Ig Type | Rabbit IgG |
| Clone | 9L258 |
| Reactivity | Human, Mouse, Rat |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate -All lanes: GRB2 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 26, 21 kDa -Observed band size: 28 kDa 2. IHC image of TMAH-00511 diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA, WB, IHC |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Cytoplasm. Endosome. Golgi apparatus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. Does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human GRB2 |
| Antigen Species | Human |
| Gene ID | 2885 |
| Uniprot ID | |
| Biology Area | Cancer, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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