Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-SMAC Antibody (1X374)
😃Good
Catalog No. TMAY-00652
Alias SMAC, diablo, IAP-binding mitochondrial protein, DFNA64
Anti-SMAC Antibody (1X374) is a Rabbit antibody targeting SMAC. Anti-SMAC Antibody (1X374) can be used in WB,ELISA,FCM,ICC/IF.
Anti-SMAC Antibody (1X374)
Anti-SMAC Antibody (1X374)
😃Good
Catalog No. TMAY-00652Alias SMAC, diablo, IAP-binding mitochondrial protein, DFNA64
Anti-SMAC Antibody (1X374) is a Rabbit antibody targeting SMAC. Anti-SMAC Antibody (1X374) can be used in WB,ELISA,FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $270 | 7-10 days |
Bulk & Custom
Add to Cart
Questions
View MoreContact us for more batch information
Resource Download
Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-SMAC Antibody (1X374) is a Rabbit antibody targeting SMAC. Anti-SMAC Antibody (1X374) can be used in WB,ELISA,FCM,ICC/IF. |
| Alias | SMAC, diablo, IAP-binding mitochondrial protein, DFNA64 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 1X374 |
| Reactivity | Human |
| Verified Activity | 1. Anti-Diablo rabbit monoclonal antibody at 1:10000 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: A431 Whole Cell Lysate. -Lane D: MCF7 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:22 kDa. -Observed band size:20 kDa. 2. Immunofluorescence staining of Human Diablo in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Diablo monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 3. Flow cytometric analysis of Human Diablo expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human Diablo (Filled hisgram), then a FITC-conjugated second step antibody. To demonstrate specificity of staining, the binding by Anti-SMAC Antibody was blocked by preincubation of the purified antibody with 20ug recombinant human Diablo for 1 hour (Black solid line hisgram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | WB,ELISA,FCM,ICC/IF |
| Recommended Dose | WB: 1:10000-1:50000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human SMAC / Diablo (rh SMAC / Diablo; TMPY-00924; NP_063940.1; Ala 56-Asp 239). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human SMAC / Diablo protein (TMPY-00924) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
Sci Citations
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Preview
Related Tags: buy Anti-SMAC Antibody (1X374) | purchase Anti-SMAC Antibody (1X374) | Anti-SMAC Antibody (1X374) cost | order Anti-SMAC Antibody (1X374)
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.