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Synonyms:

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $95 | 7-10 days | 7-10 days | |
| 10 μg | $155 | 7-10 days | 7-10 days | |
| 20 μg | $257 | 7-10 days | 7-10 days | |
| 50 μg | $497 | 7-10 days | 7-10 days | |
| 100 μg | $987 | 7-10 days | 7-10 days |
| Bioactivity | Immobilized human ACE2 protein (Fc tag) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 BA.2.75.2(Omicron)Spike S1+S2 trimer Protein(ECD,His Tag) , the EC50 is 5-15 ng/mL. |
| Description | SARS-CoV-2 BA.2.75.2 (Omicron) Spike S1+S2 trimer Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 136.41 kDa and the accession number is YP_009724390.1. |
| Species | SARS-CoV-2 |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | YP_009724390.1 |
| Construction | A DNA sequence encoding the SARS-CoV-2 (BA.2.75.2) Spike S1+S2 (YP_009724390.1, with mutations T19I, L24S, del25-27, G142D, K147E, W152R, F157L, I210V, V213G, G257S, G339H, R346T, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, G446S, N460K, S477N, T478K, E484A, F486S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, F817P, A892P, A899P, A942P, Q954H, N969K, K986P, V987P, D1199N and furin cleavage site mutants) expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.2.75.2). Predicted N terminal: Val 16 |
| Protein Purity | > 90 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a sodium citrate buffer system at pH 6. 0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Research Background |
| Molecular Weight | 136.41 kDa (predicted) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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