EGFR Protein, Human, Recombinant (Isoform Viii, hFc)

Catalog No. TMPY-05258

EGFR Protein, Human, Recombinant (Isoform Viii, hFc) is expressed in Baculovirus insect cells with hFc tag. The predicted molecular weight is 65.1 kDa and the accession number is NP_001333870.1.

EGFR Protein, Human, Recombinant (Isoform Viii, hFc)

Catalog No. TMPY-05258

EGFR Protein, Human, Recombinant (Isoform Viii, hFc) is expressed in Baculovirus insect cells with hFc tag. The predicted molecular weight is 65.1 kDa and the accession number is NP_001333870.1.

Pack Size	Price	Availability	Quantity
50 μg	$398	7-10 days
1 mg	$3,910	7-10 days

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User Guide of Recombinant Proteins

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Product Information

Biological Activity	1. Immobilized EGF Protein, Human, Recombinant (ECD), HPLC-verified at 2 μg/mL (100 μL/well) can bind EGFR Protein, Human, Recombinant (Isoform Viii, hFc Tag) in functional ELISA. 2. Immobilized Anti-EGFR(MK)-IgG1 Antibody (Cetuximab) at 2 μg/mL (100 μL/well) can bind EGFR Protein, Human, Recombinant (Isoform Viii, hFc Tag), the EC50 is 4-13 ng/mL.
Description	EGFR Protein, Human, Recombinant (Isoform Viii, hFc) is expressed in Baculovirus insect cells with hFc tag. The predicted molecular weight is 65.1 kDa and the accession number is NP_001333870.1.
Species	Human
Expression System	Baculovirus Insect Cells
Tag	C-hFc
Accession Number	NP_001333870.1
Synonyms	PIG61,NISBD2,mENA,HER1,ERBB1,ERBB,epidermal growth factor receptor
Construction	A DNA sequence encoding the human EGFR (NP_001333870.1) (Met1-Lys375) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Leu 25
Protein Purity	> 95 % as determined by SDS-PAGE.
Molecular Weight	65.1 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM Glycine, 10 mM NaCl, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy