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IL-10 Protein, Human, Recombinant V2

(Synonyms: TGIF, Interleukin-10, interleukin 10, IL10A, IL-10, GVHDS, CSIF) Copy Product Info

Synonyms: TGIF, Interleukin-10, interleukin 10, IL10A, IL-10, GVHDS, CSIF

Catalog No. TMPY-03547U Copy Product Info
IL-10 Protein, Human, Recombinant V2 is expressed in HEK293 Cells. The accession number is P22301.
IL-10 Protein, Human, Recombinant V2
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Pack SizePriceUSA StockGlobal StockQuantity
5 μg$68-In Stock
10 μg$108-In Stock
20 μg$177-In Stock
50 μg$2987-10 days7-10 days
100 μg$447-In Stock
200 μg$7917-10 days7-10 days
500 μgPreferential7-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Measured by its ability to inhibit IL-6 secretion by RAW264.7 cells. The ED50 for this effect is 0.1 -1.0 ng/mL
Immobilized Human IL-10, No Tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 61.9 ng/mL determined by ELISA.
Human IL-10 R alpha, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-10, No Tag with an affinity constant of 0.255 nM as determined in SPR assay (Biacore T200).
Description
IL-10 Protein, Human, Recombinant V2 is expressed in HEK293 Cells. The accession number is P22301.
Species
Human
Expression System
HEK293 Cells
TagTag Free
Accession NumberP22301
ConstructionSer19-Asn178
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
ReconstitutionReconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsTGIF, Interleukin-10, interleukin 10, IL10A, IL-10, GVHDS, CSIF
Research Background
Chemical Properties
Molecular Weight18.8 kDa (Predicted and reducing conditions)
Storage & Solubility Information
StorageStore at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: IL-10 Protein, Human, Recombinant V2 chemical structure | IL-10 Protein, Human, Recombinant V2 molecular weight