IL-10 Protein, Human, Recombinant V2

Name: IL-10 Protein, Human, Recombinant V2
Brand: TargetMol
SKU: TMPY-03547U
Price: 791 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: TGIF, Interleukin-10, interleukin 10, IL10A, IL-10, GVHDS, CSIF) Copy Product Info

Synonyms: TGIF, Interleukin-10, interleukin 10, IL10A, IL-10, GVHDS, CSIF

Catalog No. TMPY-03547U Copy Product Info

IL-10 Protein, Human, Recombinant V2 is expressed in HEK293 Cells. The accession number is P22301.

Customer service consultation

Pack Size	Price	USA Stock	Global Stock
5 µg	$68	-	In Stock
10 µg	$108	-	In Stock
20 µg	$177	-	In Stock
50 µg	$298	7-10 days	7-10 days
100 µg	$447	-	In Stock
200 µg	$791	7-10 days	7-10 days
500 µg	Preferential	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its ability to inhibit IL-6 secretion by RAW264.7 cells. The ED50 for this effect is 0.1 -1.0 ng/mL Immobilized Human IL-10, No Tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 61.9 ng/mL determined by ELISA. Human IL-10 R alpha, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-10, No Tag with an affinity constant of 0.255 nM as determined in SPR assay (Biacore T200).
Description	IL-10 Protein, Human, Recombinant V2 is expressed in HEK293 Cells. The accession number is P22301.
Species	Human
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	P22301
Construction	Ser19-Asn178
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	TGIF, Interleukin-10, interleukin 10, IL10A, IL-10, GVHDS, CSIF
Research Background	Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.

Chemical Properties

Molecular Weight

18.8 kDa (Predicted and reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.