Immobilized Human IL-10, No Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 21.8 ng/ml determined by ELISA (QC Test).
Human IL-10 R alpha, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-10, No Tag with an affinity constant of 0.255 nM as determined in SPR assay (Biacore T200).

IL-10 Protein, Human, Recombinant V2 is expressed in HEK293 Cells. The accession number is P22301.

Human

HEK293 Cells

TGIF,Interleukin-10,interleukin 10,IL10A,IL-10,GVHDS,CSIF

Ser19-Asn178

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.