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Synonyms: TGIF, Interleukin-10, interleukin 10, IL10A, IL-10, GVHDS, CSIF


| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $68 | - | In Stock | |
| 10 μg | $108 | - | In Stock | |
| 20 μg | $177 | - | In Stock | |
| 50 μg | $298 | 7-10 days | 7-10 days | |
| 100 μg | $447 | - | In Stock | |
| 200 μg | $791 | 7-10 days | 7-10 days | |
| 500 μg | Preferential | 7-10 days | 7-10 days |
| Bioactivity | Measured by its ability to inhibit IL-6 secretion by RAW264.7 cells. The ED50 for this effect is 0.1 -1.0 ng/mL Immobilized Human IL-10, No Tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 61.9 ng/mL determined by ELISA. Human IL-10 R alpha, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-10, No Tag with an affinity constant of 0.255 nM as determined in SPR assay (Biacore T200). |
| Description | IL-10 Protein, Human, Recombinant V2 is expressed in HEK293 Cells. The accession number is P22301. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | Tag Free |
| Accession Number | P22301 |
| Construction | Ser19-Asn178 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | TGIF, Interleukin-10, interleukin 10, IL10A, IL-10, GVHDS, CSIF |
| Research Background |
| Molecular Weight | 18.8 kDa (Predicted and reducing conditions) |
| Storage | Store at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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