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Anti-HDAC2 Antibody (4L672)
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Catalog No. TMAH-00534
Alias Histone deacetylase 2, HDAC 2, HD2, EC 3.5.1.98
Anti-HDAC2 Antibody (4L672) is an antibody targeting HDAC2. Anti-HDAC2 Antibody (4L672) can be used in ELISA, WB, IHC.
Anti-HDAC2 Antibody (4L672)
Anti-HDAC2 Antibody (4L672)
😃Good
Catalog No. TMAH-00534Alias Histone deacetylase 2, HDAC 2, HD2, EC 3.5.1.98
Anti-HDAC2 Antibody (4L672) is an antibody targeting HDAC2. Anti-HDAC2 Antibody (4L672) can be used in ELISA, WB, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC Reactivity: Human |
| Alias | Histone deacetylase 2, HDAC 2, HD2, EC 3.5.1.98 |
| Ig Type | Rabbit IgG |
| Clone | 4L672 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate -All lanes: HDAC2 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 56, 52 kDa -Observed band size: 60 kDa 2. IHC image of TMAH-00534 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA, WB, IHC |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Cytoplasm. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human HDAC2 |
| Antigen Species | Human |
| Gene ID | 3066 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Cardiovascular, Stem cells |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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