Antibody Type: Mouse Monoclonal

Application: ELISA, WB, IHC, IF, FCM

Reactivity: Human, Mouse

IgG2b

9K94

Human, Mouse

1. Western Blot
-Positive WB detected in: NFE2L2 antibody at 1:1000
-Lane 1: Hela whole cell lysate
-Lane 2: THP-1 whole cell lysate
-Lane 3: HepG2 whole cell lysate
-Lane 4: NIH/3T3 whole cell lysate
-Lane 5: RAW264.7 whole cell lysate
-Lane 6: K562 whole cell lysate
-Secondary: Goat polyclonal to Mouse IgG at 1/20000 dilution
-Predicted band size: 68 KDa
-Observed band size: 68-100 KDa
-Exposure time: 1min
2. IHC image of TMAH-00813 diluted at 1:100 and staining in paraffin-embedded human breast cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight, and detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00813 diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG labeled by HRP and visualized using 0.05% DAB.
4. Immunofluorescence staining of NIH/3T3 cells with TMAH-00813 at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
5. Immunofluorescence staining of HepG2 cells with TMAH-00813 at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
6. Overlay Peak curve showing Hela cells stained with TMAH-00813 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
7. Overlay Peak curve showing HepG2 cells stained with TMAH-00813 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IHC, IF, FCM

Monoclonal

Unconjugated

Recombinant Protein: Human Nuclear factor erythroid 2-related factor 2 Protein (256-605AA)

Human

4780

Signal Transduction