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Anti-MERS-CoV Nucleocapsid Antibody (1U169) is a Mouse antibody targeting MERS-CoV Nucleocapsid. Anti-MERS-CoV Nucleocapsid Antibody (1U169) can be used in WB,ELISA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μL | $94 | 7-10 days | |
100 μL | $294 | 7-10 days |
Description | Anti-MERS-CoV Nucleocapsid Antibody (1U169) is a Mouse antibody targeting MERS-CoV Nucleocapsid. Anti-MERS-CoV Nucleocapsid Antibody (1U169) can be used in WB,ELISA. |
Ig Type | Monoclonal Mouse IgG1 |
Clone | 1U169 |
Verified Activity | Anti-MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP mouse monoclonal antibody at 1:1000 -Sample:MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP -Lane A: 20ng -Lane B: 5ng -Lane C: 2ng -Secondary -Rabbit Anti-Mouse IgGF (ab)2/HRP at 1/10000 dilution.Performed under reducing conditions. |
Application | WB,ELISA |
Recommended Dose | WB: 1:1000-1:5000 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP (TMPY-03423; AFS88943.1; Met 1-Asp413). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
Conjucates | Unconjugated |
Immunogen | Recombinant MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP protein (TMPY-03423) |
Antigen Species | MERS-CoV |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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