CEACAM5, also known as CEA or D66e, belongs to the large CEACAM subfamily of the immunoglobulin superfamily. CEACAM5 is expressed primarily by epithelial cells, and is synthesized as a glycoprotein with an MW of 180 kDa comprising 60% carbohydrate. CEACAM5 contains one Ig-like V-type domain at the N-terminus, followed by six Ig-like C2-type domains and a GPI anchor, and exists as a homodimer. CEACAM5 and CEACAM6 are overexpressed in many cancers and are associated with adhesion and invasion. CEACAM5 can mediate cell-cell adhesion through homotypic and heterotypic interactions. It functions as a homotypic intercellular adhesion molecule and serves as a widely used tumor marker, since it is expressed at higher levels in tumorous tissues than in corresponding normal tissues. CEACAM5 has also been shown to contribute to tumorigenicity by inhibiting cellular differentiation. In addition, CEACAM5 is identified as the host receptor for the Dr family of adhesins of E.Coli, and the binding of E.coli Dr adhesins leads to dissociation of the CEACAM5 homodimer.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy
Pack Size | Availability | Price/USD | Quantity |
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100 μg | In stock | $ 386.00 | |
200 μg | 5 days | $ 682.00 | |
500 μg | 5 days | $ 1,440.00 |
Biological Information |
1. Immobilized Anti-CEA Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Human CEACAM5/CD66e Protein (His Tag), the EC50 is 5.5-16 ng/mL. 2. Loaded Anti-Human CEACAM5 antibody, human IgG1 on ProA Biosensor, can bind Recombinant Human CEACAM5 Protein, His Tag with an affinity constant of 3.72 nM as determined in a BLI assay (Routinely tested).
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Description | CEACAM5, also known as CEA or D66e, belongs to the large CEACAM subfamily of the immunoglobulin superfamily. CEACAM5 is expressed primarily by epithelial cells, and is synthesized as a glycoprotein with an MW of 180 kDa comprising 60% carbohydrate. CEACAM5 contains one Ig-like V-type domain at the N-terminus, followed by six Ig-like C2-type domains and a GPI anchor, and exists as a homodimer. CEACAM5 and CEACAM6 are overexpressed in many cancers and are associated with adhesion and invasion. CEACAM5 can mediate cell-cell adhesion through homotypic and heterotypic interactions. It functions as a homotypic intercellular adhesion molecule and serves as a widely used tumor marker, since it is expressed at higher levels in tumorous tissues than in corresponding normal tissues. CEACAM5 has also been shown to contribute to tumorigenicity by inhibiting cellular differentiation. In addition, CEACAM5 is identified as the host receptor for the Dr family of adhesins of E.Coli, and the binding of E.coli Dr adhesins leads to dissociation of the CEACAM5 homodimer.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
Species | Human |
Expression System | HEK293 |
Tag | His |
Accession Number | A0A024R0K5 |
Synonyms | CD66e, CEA, carcinoembryonic antigen-related cell adhesion molecule 5 |
Construction | A DNA sequence encoding the mature form of human CEACAM5 (NP_004354.2) (Met 1-Ala 685) was fused with a polyhistidine tag at the C-terminus. |
Protein Purity |
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
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Molecular Weight | 72.8 kDa (predicted) |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 8.0. Pleasecon tact usfor any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Reconstitution | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | CEACAM5, also known as CEA or D66e, belongs to the large CEACAM subfamily of the immunoglobulin superfamily. CEACAM5 is expressed primarily by epithelial cells, and is synthesized as a glycoprotein with an MW of 180 kDa comprising 60% carbohydrate. CEACAM5 contains one Ig-like V-type domain at the N-terminus, followed by six Ig-like C2-type domains and a GPI anchor, and exists as a homodimer. CEACAM5 and CEACAM6 are overexpressed in many cancers and are associated with adhesion and invasion. CEACAM5 can mediate cell-cell adhesion through homotypic and heterotypic interactions. It functions as a homotypic intercellular adhesion molecule and serves as a widely used tumor marker, since it is expressed at higher levels in tumorous tissues than in corresponding normal tissues. CEACAM5 has also been shown to contribute to tumorigenicity by inhibiting cellular differentiation. In addition, CEACAM5 is identified as the host receptor for the Dr family of adhesins of E.Coli, and the binding of E.coli Dr adhesins leads to dissociation of the CEACAM5 homodimer.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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Please read the User Guide of Recombinant Proteins for more specific information.
CEACAM5 Protein, Human, Recombinant (His) CD66e CEA carcinoembryonic antigen-related cell adhesion molecule 5 recombinant recombinant-proteins proteins protein