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Anti-ASS1 Antibody (4Q430)

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Catalog No. TMAH-00083
Alias Citrulline--Aspartate Ligase, ASS1, ASS, Argininosuccinate Synthase

Anti-ASS1 Antibody (4Q430) is an antibody targeting ASS1. Anti-ASS1 Antibody (4Q430) can be used in ELISA, IHC, IF, FCM.

Anti-ASS1 Antibody (4Q430)

Anti-ASS1 Antibody (4Q430)

😃Good
Catalog No. TMAH-00083Alias Citrulline--Aspartate Ligase, ASS1, ASS, Argininosuccinate Synthase
Anti-ASS1 Antibody (4Q430) is an antibody targeting ASS1. Anti-ASS1 Antibody (4Q430) can be used in ELISA, IHC, IF, FCM.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$348 7-10 days
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Product Introduction

Bioactivity
Description
Anti-ASS1 Antibody (4Q430) is an antibody targeting ASS1. Anti-ASS1 Antibody (4Q430) can be used in ELISA, IHC, IF, FCM.
AliasCitrulline--Aspartate Ligase, ASS1, ASS, Argininosuccinate Synthase
Ig Type
Rabbit IgG
Clone
4Q430
Reactivity
Human
Verified Activity
1. IHC image of TMAH-00083 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.56% DAB.
2. Immunofluorescence staining of Hela with TMAH-00083 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 516-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
3. Overlay Peak curve showing Jurkat cells stained with TMAH-00083 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Application
ELISA, IHC, IF, FCM
Recommended Dose
IHC:1:50-1:200; IF:1:50-1:200; FCM:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCytoplasm, cytosol.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundOne of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human ASS1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cancer, Metabolism, Signal transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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