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Anti-ER beta Polyclonal Antibody

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Catalog No. TMAB-00632
Alias Nuclear receptor subfamily 3 group A member 2, NR3A2, Estrogen receptor beta, ESTRB, ESR2, ER-beta

Anti-ER beta Polyclonal Antibody is a Rabbit antibody targeting ER beta. Anti-ER beta Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA.

Anti-ER beta Polyclonal Antibody

Anti-ER beta Polyclonal Antibody

😃Good
Catalog No. TMAB-00632Alias Nuclear receptor subfamily 3 group A member 2, NR3A2, Estrogen receptor beta, ESTRB, ESR2, ER-beta
Anti-ER beta Polyclonal Antibody is a Rabbit antibody targeting ER beta. Anti-ER beta Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3747-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-ER beta Polyclonal Antibody is a Rabbit antibody targeting ER beta. Anti-ER beta Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA.
AliasNuclear receptor subfamily 3 group A member 2, NR3A2, Estrogen receptor beta, ESTRB, ESR2, ER-beta
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Sample:
Kidney (Mouse) lysate at 30 μg;
Brain (Rat) lysate at 30 μg;
Primary: Anti-ER-beta (TMAB-00632) at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit Igg (secondary antibody) at 1: 3000;
Predicted band size: 58 kDa
Observed band size: 58 kDa
2. Sample:
Lane 1: Testis (Mouse) Lysate at 40 μg
Lane 2: Testis (Rat) Lysate at 40 μg
Lane 3: Ovary (Mouse) Lysate at 40 μg
Lane 4: Uterus (Mouse) Lysate at 40 μg
Lane 5: Uterus (Rat) Lysate at 40 μg
Primary: Anti-Estrogen receptor beta (TMAB-00632) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58-62 kDa
3. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (TMAB-00632) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
13. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Cerebellum tissue lysates
Lane 4: Rat Testis tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Rat Cerebellum tissue lysates
Lane 7: Human MCF-7 cell lysates
Lane 8: Human Jurkat cell lysates
Lane 9: Human U-2 OS cell lysates
Lane 10: Human A431 cell lysates
Primary: Anti-ESR2 (TMAB-00632) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 63 kDa
14. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (Estrogen Receptor beta) polyclonal Antibody, unconjugated (TMAB-00632) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green ) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA
Recommended Dose
WB: 1:500-2000; IHC-P: 1:400-800; IHC-Fr: 1:400-800; ICC/IF: 1:50-200; IF: 1:100-500; ELISA: 1:5000-10000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a member of the family of estrogenreceptors and superfamily of nuclear receptor transcriptionfactors. The gene product contains an N-terminal DNA binding domainand C-terminal ligand binding domain and is localized to thenucleus, cytoplasm, and mitochondria. Upon binding to17beta-estradiol or related ligands, the encoded protein formshomo- or hetero-dimers that interact with specific DNA sequences toactivate transcription. Some isoforms dominantly inhibit theactivity of other estrogen receptor family members. Severalalternatively spliced transcript variants of this gene have beendescribed, but the full-length nature of some of these variants hasnot been fully characterized. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human ER-beta
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Estrogen,Receptors,ChIP kits,Estrogen,Zinc Finger,Gonadotrophic axis,Estrogen
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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