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Anti-TRIM21 Polyclonal Antibody
😃Good
Catalog No. TMAB-01896
Alias tripartite motif containing 21, SSA1, SSA, RO52, Ro/SSA, RNF81
Anti-TRIM21 Polyclonal Antibody is a Rabbit antibody targeting TRIM21. Anti-TRIM21 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Anti-TRIM21 Polyclonal Antibody
Anti-TRIM21 Polyclonal Antibody
😃Good
Catalog No. TMAB-01896Alias tripartite motif containing 21, SSA1, SSA, RO52, Ro/SSA, RNF81
Anti-TRIM21 Polyclonal Antibody is a Rabbit antibody targeting TRIM21. Anti-TRIM21 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-TRIM21 Polyclonal Antibody is a Rabbit antibody targeting TRIM21. Anti-TRIM21 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
| Alias | tripartite motif containing 21, SSA1, SSA, RO52, Ro/SSA, RNF81 |
| Ig Type | IgG |
| Reactivity | Human,Rat,Mouse |
| Verified Activity | 1. Sample: U937 Cell Lysate at 40 μg Primary: Anti-TRIM21 (TMAB-01896) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 56 kDa 2. Sample: Heart (Mouse) Lysate at 40 μg Primary: Anti-TRIM21 (TMAB-01896) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa 3. Sample: Lung (Mouse) Lysate at 40 μg Primary: Anti-TRIM21 (TMAB-01896) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa 4. Paraformaldehyde-fixed, paraffin embedded (mouse lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TRIM21) Polyclonal Antibody, Unconjugated (TMAB-01896) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TRIM21) Polyclonal Antibody, Unconjugated (TMAB-01896) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TRIM21) Polyclonal Antibody, Unconjugated (TMAB-01896) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (TRIM21) Polyclonal Antibody, Unconjugated (TMAB-01896) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Cytoplasm, P-body. Note=Enters the nucleus upon exposure to nitric oxide. Localizes to small dot- or rod-like structures in the cytoplasm, called cytoplasmic bodies (P-body) that are located underneath the plasma membrane and also diffusely in the cytoplasm and are highly motil in cells. Cytoplasmic bodies are located along the microtubules and do not share the same cytoplasmic bodies with TRIM5. Colocalizes with DCP2 in P-body. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human TRIM21 N-terminus |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | DNA / RNA binding,RNA Processing,Splicing,Antiviral Signaling |
| Molecular Weight | Theoretical: 52 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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