Antibody Type: Mouse Monoclonal

Application: ELISA, WB, IHC, IF, FC, IP

Reactivity: Human, Mouse, Rat, Rabbit

IgG1

1H358

Human, Mouse, Rat, Rabbit

1. Western Blot
-Positive WB detected in: K562 whole cell lysate, NIH/3T3 whole cell lysate, Rat Brain tissue, Mouse Brain tissue, Rabbit Skeletal Muscle tissue, Rat Kidney tissue, Rabbit Kidney tissue
-All lanes ENO1 antibody at 1:10000
-Secondary: Goat polyclonal to mouse IgG at 1/10000 dilution
-Predicted band size: 47 KDa
-Observed band size: 47 KDa
-Exposure time: 1min
2. Western Blot
-Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate
-All lanes ENO1 antibody at 1:10000
-Secondary: Goat polyclonal to mouse IgG at 1/10000 dilution
-Predicted band size: 47 KDa
-Observed band size: 47 KDa
-Exposure time: 10s
3. Western Blot
-Positive WB detected in: HepG2 whole cell lysate at 20µg, 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg
-All lanes: ENO1 antibody at 1:5000
-Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution
-Predicted band size: 47 kDa
-Observed band size: 47 KDa
-Exposure time: 10s
4. Western Blot
-Positive WB detected in: MCF-7 whole cell lysate
-All lanes: ENO1 antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000
-Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution
-Predicted band size: 47 kDa
-Observed band size: 47 KDa
-Exposure time: 10s
5. IHC image of TMAH-00385 diluted at 1:500 and staining in paraffin-embedded human liver cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
6. IHC image of TMAH-00385 diluted at 1:500 and staining in paraffin-embedded human liver cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
7. IHC image of TMAH-00385 diluted at 1:500 and staining in paraffin-embedded human colon cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
8. IHC image of TMAH-00385 diluted at 1:500 and staining in paraffin-embedded human colon cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
9. IHC image of TMAH-00385 diluted at 1:500 and staining in paraffin-embedded human pancreas tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
10. IHC image of TMAH-00385 diluted at 1:500 and staining in paraffin-embedded human pancreas tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
11. Immunofluorescence staining of MCF-7 cells with TMAH-00385 at 1:130, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
12. Immunofluorescence staining of Hela cells with TMAH-00385 at 1:130, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
13. Immunofluorescence staining of HepG2 cells with TMAH-00385 at 1:130, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
14. Overlay histogram showing Hela cells stained with TMAH-00385 (red line) at 1:260. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
15. Overlay histogram showing MCF-7 cells stained with TMAH-00385 (red line) at 1:260. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
16. Immunoprecipitating ENO1 in HepG2 whoanle cell lysate
-Lane 1: Mouse control IgG (1µg) instead of TMAH-00385 in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
-Lane 2: TMAH-00386 (2µl) + HepG2 whole cell lysate (500µg)
-Lane 3: HepG2 whole cell lysate (10µg)
17. 1-Exosomes extracted from plasma
2-Exosomes extracted from serum
3-Exosomes extracted from urine
4-Exosomes extracted from Hela cells
5-Exosomes extracted from latex
6-Exosomes extracted from saliva
18. 1-Exosomes extracted from MG63 cells
2-Exosomes extracted from Ntera-2 cells
3-MG63 cell Lysate
19. 1-Exosomes extracted from HEPG2 cells
2-Exosomes extracted from PC-3 cells
3-Exosomes extracted from Hela cells
4-Exosomes extracted from U87 cells
5-Hela cell Lysate
20. 1-Exosomes extracted from Raji cells
2-Exosomes extracted from U251 cells
3-Raji cell Lysate

ELISA, WB, IHC, IF, FC, IP

Monoclonal

Unconjugated

Recombinant Protein: Human Alpha-enolase Protein (2-434AA)

Human

2023

Immunology