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Anti-Latexin Antibody (7N890)
😃Good
Catalog No. TMAY-00472
Alias latexin
Anti-Latexin Antibody (7N890) is a Mouse antibody targeting Latexin. Anti-Latexin Antibody (7N890) can be used in WB,ELISA,IP.
Anti-Latexin Antibody (7N890)
Anti-Latexin Antibody (7N890)
😃Good
Catalog No. TMAY-00472Alias latexin
Anti-Latexin Antibody (7N890) is a Mouse antibody targeting Latexin. Anti-Latexin Antibody (7N890) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Latexin Antibody (7N890) is a Mouse antibody targeting Latexin. Anti-Latexin Antibody (7N890) can be used in WB,ELISA,IP. |
| Alias | latexin |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 7N890 |
| Reactivity | Human |
| Verified Activity | 1. Anti-LXN mouse monoclonal antibody at 1:500 dilution. -Lane A: A549 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:26 kDa. -Observed band size:26 kDa. 2. LXN was immunoprecipitated using: -Lane A:0.5 mg MCF-7 Whole Cell Lysate -0.5 µL anti-LXN mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-LXN mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 25.7 kDa. -Observed band size: 27 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:1000; ELISA: 1:5000-1:10000; IP: 1-2 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Latexin (rh Latexin; TMPY-00870; NP_064554.3; Glu 2-Glu 222). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI, and LXN, is a cytoplasm protein that belongs to the protease inhibitor I47 (latexin) family. It is highly expressed in the heart, prostate, ovary, kidney, pancreas, and colon. Latexin / LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in the human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including the brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. Also, Latexin is implicated in Alzheimer's disease. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human Latexin protein (TMPY-00870) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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