TRAIL R2/DR5/TNFRSF10B Protein, Human, Recombinant (hFc)

DR5, also called TRAIL R2, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). DR5 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.

Pack Size	Availability	Price/USD
100 μg	5 days	$ 347.00
500 μg	5 days	$ 1,400.00
1 mg	5 days	$ 2,330.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 347.00

500 μg

5 days

$ 1,400.00

1 mg

5 days

$ 2,330.00

Description

Species	Human
Expression System	HEK293
Tag	C-hFc
Accession Number	O14763-1
Synonyms	TRAIL R2, TNFRSF10B, TRICK2, KILLER, DR5, TRAIL receptor 2, Fas-like protein, TRICKB, ZTNFR9, DR5TRICK2B, CD262, TRICK2A, KILLER/DR5, TRAILR2
Construction	Recombinant Human TRAIL R2/DR5/TNFRSF10B Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ile56-Glu182.[Accession \|O14763-1]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	The protein has a predicted MW of 41.1 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per ug by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in 50mM Tris, 100mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	DR5, also called TRAIL R2, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). DR5 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.