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Anti-CD44 Antibody (4K229)
😃Good
Catalog No. TMAY-02284
Alias Pgp1, MIC4, MDU3, MDU2, MC56, LHR, IN, HUTCH-I, HCELL, ECMR-III, CSPG8, CDW44, CD44 molecule (Indian blood group)
Anti-CD44 Antibody (4K229) is a Mouse antibody targeting CD44. Anti-CD44 Antibody (4K229) can be used in WB,ELISA,ELISA(Det),IP.
Anti-CD44 Antibody (4K229)
Anti-CD44 Antibody (4K229)
😃Good
Catalog No. TMAY-02284Alias Pgp1, MIC4, MDU3, MDU2, MC56, LHR, IN, HUTCH-I, HCELL, ECMR-III, CSPG8, CDW44, CD44 molecule (Indian blood group)
Anti-CD44 Antibody (4K229) is a Mouse antibody targeting CD44. Anti-CD44 Antibody (4K229) can be used in WB,ELISA,ELISA(Det),IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $69 | 7-10 days | |
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CD44 Antibody (4K229) is a Mouse antibody targeting CD44. Anti-CD44 Antibody (4K229) can be used in WB,ELISA,ELISA(Det),IP. |
| Alias | Pgp1, MIC4, MDU3, MDU2, MC56, LHR, IN, HUTCH-I, HCELL, ECMR-III, CSPG8, CDW44, CD44 molecule (Indian blood group) |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 4K229 |
| Reactivity | Human |
| Verified Activity | 1. CD44 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate -0.5 µL anti-CD44 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-CD44 mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 82 kDa. -Observed band size: 98 kDa. 2. Anti-CD44 mouse monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:82 kDa. -Observed band size:98 kDa |
| Application | WB,ELISA,ELISA(Det),IP |
| Recommended Dose | WB: 1:500-1:1000; ELISA: 1:5000-1:10000; IP: 0.2-1 μL/mg of lysate; ELISA(Det): 1:5000-1:50000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD44 (rh CD44; TMPY-02033; P16070-1; Met 1-Pro 220). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | CD44 is a type I transmembrane protein and a member of the cartilage link protein family. It is involved in cell-cell and cell-matrix interactions and signal transduction. Several CD44 ligands have been identified. The most extensively characterized ligand for CD44 is hyaluronan, a component of the extracellular matrix. CD44 protein is expressed on the majority of immune cells. The binding of CD44 to hyaluronan is induced on T lymphocytes after activation by antigen and on monocytes after stimulation by inflammatory agents. Under inflammatory conditions, CD44 on endothelial cells presents hyaluronan to CD44 on activated T lymphocytes and mediates a rolling interaction under flow conditions. Perturbations of the hyaluronan-CD44 interaction at the plasma membrane by various antagonists result in attenuation of receptor tyrosine kinase and transporter activities and inhibition of tumor progression in vivo. CD44 is known to interact with the ezrin family (ERM family) members and form a complex that plays diverse roles within both normal and abnormal cells, particularly cancer cells. CD44 and ezrin and their respective complex have properties suggesting that they may be important in the process of tumour-endothelium interactions, cell migrations, cell adhesion, tumour progression and metastasis. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human CD44 protein (TMPY-02033) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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