Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-Beta-Catenin Antibody (8B317)

😃Good
Catalog No. TMAY-01820
Alias β-Catenin, Mesc, Catnb, catenin β1, catenin beta 1, Bfc

Anti-Beta-Catenin Antibody (8B317) is a Rabbit antibody targeting Beta-Catenin. Anti-Beta-Catenin Antibody (8B317) can be used in ELISA,IHC-P,FCM,ICC/IF.

Anti-Beta-Catenin Antibody (8B317)

Anti-Beta-Catenin Antibody (8B317)

😃Good
Catalog No. TMAY-01820Alias β-Catenin, Mesc, Catnb, catenin β1, catenin beta 1, Bfc
Anti-Beta-Catenin Antibody (8B317) is a Rabbit antibody targeting Beta-Catenin. Anti-Beta-Catenin Antibody (8B317) can be used in ELISA,IHC-P,FCM,ICC/IF.
Pack SizePriceAvailabilityQuantity
100 μL255 € 7-10 days
Bulk & Custom
Add to Cart
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-Beta-Catenin Antibody (8B317) is a Rabbit antibody targeting Beta-Catenin. Anti-Beta-Catenin Antibody (8B317) can be used in ELISA,IHC-P,FCM,ICC/IF.
Aliasβ-Catenin, Mesc, Catnb, catenin β1, catenin beta 1, Bfc
Ig Type
Monoclonal Rabbit IgG
Clone
8B317
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human CTNNB1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CTNNB1 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.

2. Immunochemical staining of human CTNNB1 in human skin with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).

3. Immunochemical staining of human CTNNB1 in human colon with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).

4. Flow cytometric analysis of Human CTNNB1 (β-Catenin) expression on Hela cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CTNNB1 (β-Catenin), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
ELISA,IHC-P,FCM,ICC/IF
Recommended Dose
ELISA: 1:5000-1:10000; IHC-P: 1:100-1:500; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human beta-Catenin / CTNNB1 (rh beta-Catenin / CTNNB1; TMPY-02205; P35222-1; Met1-Leu781).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research Backgroundbeta-Catenin, also known as CTNNB1, is a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain, which is specialized for protein-protein binding. It is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. CTNNB1 also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, beta-Catenin binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Defects in beta-Catenin can cause colorectal cancer, pilomatrixoma (PTR), medulloblastoma, and ovarian cancer. CTNNB1 is a key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. CTNNB1 is involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human beta-Catenin / CTNNB1 protein (TMPY-02205)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

Sci Citations

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: buy Anti-Beta-Catenin Antibody (8B317) | purchase Anti-Beta-Catenin Antibody (8B317) | Anti-Beta-Catenin Antibody (8B317) cost | order Anti-Beta-Catenin Antibody (8B317)