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Anti-EphB4 Antibody (6A752)
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Catalog No. TMAY-00504
Anti-EphB4 Antibody (6A752) is a Mouse antibody targeting EphB4. Anti-EphB4 Antibody (6A752) can be used in ELISA,FCM,ICC/IF.
Anti-EphB4 Antibody (6A752)
Anti-EphB4 Antibody (6A752)
😃Good
Catalog No. TMAY-00504
Anti-EphB4 Antibody (6A752) is a Mouse antibody targeting EphB4. Anti-EphB4 Antibody (6A752) can be used in ELISA,FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-EphB4 Antibody (6A752) is a Mouse antibody targeting EphB4. Anti-EphB4 Antibody (6A752) can be used in ELISA,FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 6A752 |
| Reactivity | Human |
| Verified Activity | 1. Flow cytometric analysis of human EphB4 expression on MCF-7 cells. Cells were stained with purified anti-Human EphB4, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of EPhB4 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human EPhB4 monoclonal antibody (1:30) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI(blue). |
| Application | ELISA,FCM,ICC/IF |
| Recommended Dose | ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EphB4 / HTK extracellular domain (rhEphB4; TMPY-00787; NP_004435.3; Met 1-Ala 539). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers. |
| Conjucates | Unconjugated |
| Others Formats | APC/FITC/PE |
| Antibody Types Available | 4 |
| Immunogen | Recombinant Human EphB4 protein (TMPY-00787) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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