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TNF alpha Protein, Ferret, Recombinant (His)
(Synonyms: TNF-α, TNFα, TNFSF2, TNFSF1A, TNFATNF, TNF-alpha, TNFalpha, TNFA, TNF α, TNF, DIF, Cachectin, APC1 protein) Copy Product InfoSynonyms: TNF-α, TNFα, TNFSF2, TNFSF1A, TNFATNF, TNF-alpha, TNFalpha, TNFA, TNF α, TNF, DIF, Cachectin, APC1 protein
Catalog No. TMPK-00583 Copy Product Info
TNF alpha Protein, Ferret, Recombinant (His) is expressed in E. coli expression system with C-His tag. The predicted molecular weight is 18.88 kDa and the accession number is A3FBF1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $58 | 7-10 days | 7-10 days | |
| 10 μg | $92 | 7-10 days | 7-10 days | |
| 20 μg | $148 | 7-10 days | 7-10 days | |
| 50 μg | $289 | 7-10 days | 7-10 days | |
| 100 μg | $487 | 7-10 days | 7-10 days | |
| 200 μg | $883 | 7-10 days | 7-10 days | |
| 500 μg | $1,950 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Ferret TNF-alpha, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 10.2ng/ml determined by ELISA. |
| Description | TNF alpha Protein, Ferret, Recombinant (His) is expressed in E. coli expression system with C-His tag. The predicted molecular weight is 18.88 kDa and the accession number is A3FBF1. |
| Species | Ferret |
| Expression System | E. coli |
| Tag | C-His |
| Accession Number | A3FBF1 |
| Construction | Val77-Leu233 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | TNF-α, TNFα, TNFSF2, TNFSF1A, TNFATNF, TNF-alpha, TNFalpha, TNFA, TNF α, TNF, DIF, Cachectin, APC1 protein |
| Research Background | Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. |
| Molecular Weight | 18.88 kDa (predicted) same as Tris-Bis PAGE result. |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: TNF alpha Protein, Ferret, Recombinant (His) chemical structure | TNF alpha Protein, Ferret, Recombinant (His) molecular weight
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