Immobilized SARS-COV-2 Spike S1, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 45.7 ng/ml determined by ELISA.

SARS-CoV-2 Spike S1 Protein (HEK293, His) V2 is expressed in HEK293 Cells. The accession number is QHO60594.1.

SARS-COV-2

HEK293 Cells

Gln14-Arg683

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.