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TNF alpha Trimer Protein, Human, Recombinant

(Synonyms: Tumor Necrosis Factor Ligand Superfamily Member 2, tumor necrosis factor, Tumor Necrosis Factor, TNF-α, TNFα, TNFSF-2, TNFSF2, TNFSF1A, TNFSF 2, TNFATNF, TNF-alpha, TNF-Alpha, TNFalpha, TNF-a, TNFA, TNF α, TNF, DIF, Cachectin, APC1 protein) Copy Product Info

Synonyms: Tumor Necrosis Factor Ligand Superfamily Member 2, tumor necrosis factor, Tumor Necrosis Factor, TNF-α, TNFα, TNFSF-2, TNFSF2, TNFSF1A, TNFSF 2, TNFATNF, TNF-alpha, TNF-Alpha, TNFalpha, TNF-a, TNFA, TNF α, TNF, DIF, Cachectin, APC1 protein

Catalog No. TMPK-02024 Copy Product Info
TNF alpha Trimer Protein, Human, Recombinant is expressed in HEK293 Cells. The accession number is P01375.
TNF alpha Trimer Protein, Human, Recombinant
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Product Introduction

Bioactivity
Bioactivity
Immobilized Human TNF alpha, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 2.7 ng/ml determined by ELISA (QC Test).
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25-100 pg/mL (QC Test).
Description
TNF alpha Trimer Protein, Human, Recombinant is expressed in HEK293 Cells. The accession number is P01375.
Species
Human
Expression System
HEK293 Cells
TagTag Free
Accession NumberP01375
ConstructionVal77-Leu233
Protein Purity
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin< 0.1 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsTumor Necrosis Factor Ligand Superfamily Member 2, tumor necrosis factor, Tumor Necrosis Factor, TNF-α, TNFα, TNFSF-2, TNFSF2, TNFSF1A, TNFSF 2, TNFATNF, TNF-alpha, TNF-Alpha, TNFalpha, TNF-a, TNFA, TNF α, TNF, DIF, Cachectin, APC1 protein
Research Background
Chemical Properties
Molecular Weight17.35 kDa (Predicted); 18-22 kDa (Reducing condition, due to glycosylation)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: TNF alpha Trimer Protein, Human, Recombinant chemical structure | TNF alpha Trimer Protein, Human, Recombinant molecular weight