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Synonyms: Tumor Necrosis Factor Ligand Superfamily Member 2, tumor necrosis factor, Tumor Necrosis Factor, TNF-α, TNFα, TNFSF-2, TNFSF2, TNFSF1A, TNFSF 2, TNFATNF, TNF-alpha, TNF-Alpha, TNFalpha, TNF-a, TNFA, TNF α, TNF, DIF, Cachectin, APC1 protein


| Bioactivity | Immobilized Human TNF alpha, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 2.7 ng/ml determined by ELISA (QC Test). Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25-100 pg/mL (QC Test). |
| Description | TNF alpha Trimer Protein, Human, Recombinant is expressed in HEK293 Cells. The accession number is P01375. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | Tag Free |
| Accession Number | P01375 |
| Construction | Val77-Leu233 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 0.1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | Tumor Necrosis Factor Ligand Superfamily Member 2, tumor necrosis factor, Tumor Necrosis Factor, TNF-α, TNFα, TNFSF-2, TNFSF2, TNFSF1A, TNFSF 2, TNFATNF, TNF-alpha, TNF-Alpha, TNFalpha, TNF-a, TNFA, TNF α, TNF, DIF, Cachectin, APC1 protein |
| Research Background |
| Molecular Weight | 17.35 kDa (Predicted); 18-22 kDa (Reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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