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Anti-Fas/CD95 Antibody (3C638) (Synonyms: TNFRSF6, FASTM, FAS1, Fas cell surface death receptor, CD95, APT1...)
Catalog No. TMAH-00419 Copy Product Info
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Anti-Fas/CD95 Antibody (3C638) is an antibody targeting Fas/CD95. Anti-Fas/CD95 Antibody (3C638) can be used in ELISA,IHC,WB.
Anti-Fas/CD95 Antibody (3C638)
Copy Product Info🥰Excellent
Catalog No. TMAH-00419
Synonyms TNFRSF6, FASTM, FAS1, Fas cell surface death receptor, CD95, APT1, APO-1, ALPS1A
Anti-Fas/CD95 Antibody (3C638) is an antibody targeting Fas/CD95. Anti-Fas/CD95 Antibody (3C638) can be used in ELISA,IHC,WB.
Anti-Fas/CD95 Antibody
(3C638)
(3C638)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $207 | 7-10 days | 7-10 days | |
| 100 μL | $349 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
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| Description | Anti-Fas/CD95 Antibody (3C638) is an antibody targeting Fas/CD95. Anti-Fas/CD95 Antibody (3C638) can be used in ELISA,IHC,WB. |
| Synonyms | TNFRSF6, FASTM, FAS1, Fas cell surface death receptor, CD95, APT1, APO-1, ALPS1A |
| Ig Type | Rabbit IgG |
| Clone | 3C638 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate, HL60 whole cell lysate, U87 whole cell lysate -All lanes: FAS antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 38, 12, 10, 17, 15, 36, 25 kDa -Observed band size: 45 kDa 2. IHC image of TMAH-00419 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00419 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Membrane raft.; [Isoform 2]: Secreted.; [Isoform 3]: Secreted.; [Isoform 4]: Secreted.; [Isoform 5]: Secreted.; [Isoform 6]: Secreted. |
| Tissue Specificity | Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Fas |
| Antigen Species | Human |
| Gene Name | FAS |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cell biology, Immunology |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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