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Anti-PRKAG1 Antibody (6O628)
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Catalog No. TMAH-01001
Alias AMPKg, AMPK subunit gamma-1, AMPK gamma1, 5'-AMP-activated protein kinase subunit gamma-1
Anti-PRKAG1 Antibody (6O628) is an antibody targeting PRKAG1. Anti-PRKAG1 Antibody (6O628) can be used in ELISA, IHC.
Anti-PRKAG1 Antibody (6O628)
Anti-PRKAG1 Antibody (6O628)
😃Good
Catalog No. TMAH-01001Alias AMPKg, AMPK subunit gamma-1, AMPK gamma1, 5'-AMP-activated protein kinase subunit gamma-1
Anti-PRKAG1 Antibody (6O628) is an antibody targeting PRKAG1. Anti-PRKAG1 Antibody (6O628) can be used in ELISA, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-PRKAG1 Antibody (6O628) is an antibody targeting PRKAG1. Anti-PRKAG1 Antibody (6O628) can be used in ELISA, IHC. |
| Alias | AMPKg, AMPK subunit gamma-1, AMPK gamma1, 5'-AMP-activated protein kinase subunit gamma-1 |
| Ig Type | Rabbit IgG |
| Clone | 6O628 |
| Reactivity | Human |
| Verified Activity | IHC image of TMAH-01001 diluted at 1:100 and staining in paraffin-embedded human Prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA, IHC |
| Recommended Dose | IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human PRKAG1 |
| Antigen Species | Human |
| Gene ID | 5571 |
| Uniprot ID | |
| Biology Area | Cancer, Cardiovascular, Metabolism, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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