Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IHC, IF

Reactivity: Human, Mouse

Rabbit IgG

4C993

Human, Mouse

1. Western Blot
-Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate
-All lanes: CDKN1B antibody at 0.9μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 23 KDa
-Observed band size: 27 KDa
2. IHC image of TMAH-00256 diluted at 1:97.5 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
3. IHC image of TMAH-00256 diluted at 1:97.5 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
4. Immunofluorescence staining of HepG2 cells with TMAH-00256 at 1:32.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ELISA, WB, IHC, IF

WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human CDKN1B

Human

1027

Cell Biology