Antibody Type: Rabbit Monoclonal

Application: WB, ELISA, FCM, ICC/IF, IP

Reactivity: Human

Rabbit IgG

8I17

Human

1. Immunofluorescence staining of Human Galectin3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human Galectin3 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nuclear and cytoplasm.
2. Flow cytometric analysis of Human Galectin3 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human Galectin3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
3. Anti-Galectin3 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: HeLa Whole Cell Lysate.
-Lane B: SW480 Whole Cell Lysate.
-Lane C: A431 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size:26 kDa.
-Observed band size:30 kDa.
4. NKX3-1 was immunoprecipitated using:
-Lane A:0.5 mg mouse pancreas Whole Cell Lysate.
-4 µL anti-NKX3-1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
-Primary antibody:
-Anti-NKX3-1 rabbit polyclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size: 27 kDa.
-Observed band size:30 kDa

WB,ELISA,FCM,ICC/IF,IP

WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100; IP: 0.5-2 μL/mg of lysate

Monoclonal

Unconjugated

Recombinant Protein: Human Galectin-3 / LGALS3 protein

Human