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Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody
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Catalog No. TMAB-01483
Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PPAR alpha/PPARA alpha (Ser12). Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody
Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody
😃Good
Catalog No. TMAB-01483
Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PPAR alpha/PPARA alpha (Ser12). Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PPAR alpha/PPARA alpha (Ser12). Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat,Rabbit (predicted:Dog,Pig,Cow,Horse,GuineaPig) |
| Verified Activity | 1. phosphopeptide non phosphopeptide 2. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-phospho-PPAR alpha (Ser12) Polyclonal Antibody, Unconjugated (TMAB-01483) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Sample: Lane 1: Heart (Mouse) Lysate at 40 μg Lane 2: Liver (Mouse) Lysate at 40 μg Lane 3: Kidney (Mouse) Lysate at 40 μg Lane 4: Cerebrum (Mouse) Lysate at 40 μg Lane 5: Cerebrum (Rat) Lysate at 40 μg Lane 6: Jurkat (Human) Cell Lysate at 30 μg Lane 7: A431 (Human) Cell Lysate at 30 μg Primary: Anti-phospho-PPAR alpha (Ser12) (TMAB-01483) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kDa Observed band size: 50 kDa |
| Application | WB,IHC-P,IHC-Fr,IF,ELISA |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Peroxisome proliferators are nongenotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed Peroxisome Proliferator Activated Receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. |
| Immunogen | KLH conjugated synthesised phosphopeptide: human PPAR alpha around the phosphorylation site of ser12 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Metabolism of lipids and lipoproteins,Response to hypoxia,Nuclear hormone receptors,Metabolism,Zinc Finger,Fatty acids,Lipid metabolism,Mitochondrial transcription,Hypoxia,Mitochondrial Biogenesis,Fatty acid oxidation,Obesity |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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