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Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody

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Catalog No. TMAB-01483

Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PPAR alpha/PPARA alpha (Ser12). Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody

Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody

😃Good
Catalog No. TMAB-01483
Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PPAR alpha/PPARA alpha (Ser12). Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$374 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PPAR alpha/PPARA alpha (Ser12). Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Ig Type
IgG
Reactivity
Human,Mouse,Rat,Rabbit (predicted:Dog,Pig,Cow,Horse,GuineaPig)
Verified Activity
1. phosphopeptide
non phosphopeptide
2. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-phospho-PPAR alpha (Ser12) Polyclonal Antibody, Unconjugated (TMAB-01483) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane 1: Heart (Mouse) Lysate at 40 μg
Lane 2: Liver (Mouse) Lysate at 40 μg
Lane 3: Kidney (Mouse) Lysate at 40 μg
Lane 4: Cerebrum (Mouse) Lysate at 40 μg
Lane 5: Cerebrum (Rat) Lysate at 40 μg
Lane 6: Jurkat (Human) Cell Lysate at 30 μg
Lane 7: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-PPAR alpha (Ser12) (TMAB-01483) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 50 kDa
Application
WB,IHC-P,IHC-Fr,IF,ELISA
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundPeroxisome proliferators are nongenotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed Peroxisome Proliferator Activated Receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.
Antigen Details
Immunogen
KLH conjugated synthesised phosphopeptide: human PPAR alpha around the phosphorylation site of ser12
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Metabolism of lipids and lipoproteins,Response to hypoxia,Nuclear hormone receptors,Metabolism,Zinc Finger,Fatty acids,Lipid metabolism,Mitochondrial transcription,Hypoxia,Mitochondrial Biogenesis,Fatty acid oxidation,Obesity
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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